A capsule stain was preform, the results were analyzed and observed. An additional procedure that was done, was the Fast Actin staining which helps to see if the bacteria contains Mycolic acid in their cell walls, which determines the structure and function of the cytoskeleton in living and fixed cells (Shah). As expected for both E.coli and K. Pnenumia the fast acting results were negative. For both E.coli and K. Pnenumia the Oxidase test was positive a reaction was obtained. The EMB agar is also an important procedure test that helps distinguishing pathogenic microbes in a short period of time (Shah).
Abstract This experiment was carried out to determine the species of the unknown organism. Once a choice of the unknown was made a Gram stain was conducted to determine the gram nature and morphology of the organism which was Gram negative bacilli. Based on those results a citrate utilization test was performed resulting in a positive test. Following the flow chart the next test to conduct was a motility test which also had a positive outcome.
Introduction The purpose of this experiment was to apply all knowledge gained from the entire semester while in the lab and apply it to be able to identify an unknown genus and species gram positive bacteria. Each student was given a petri dish with an unknown Gram positive bacterium inside. The petri dish with the unknown gram positive bacteria that was used in my experiment was #8. The possible bacteria inside my petri dish could be any of the following: Bacillus subtilis, Bacillus cereus, Mycobacterium species, Corynebacterium species, Lactobacillus, Staphylococcus aureus, Micrococcus luteus, Staphylococcus epidermidis, Enterococcus faecalis, Streptococcus pyogenes, and Streptococcus pneumoniae Hypothesis
The membrane only allows certain molecules to pass through, water molecules are easily able to go through (Freeman 2011). Since sodium chloride is a normal saline it contains salt. The salt ions are not easily able to pass through the cell membrane like water. Compared to water, the sodium chloride should make the potatoes weigh less than in water, which should support our
It starts orange and turns yellow positive for microbe 3C Klebsiella oxytoca (Professor Brady, Personal Communication). Procedure 1 Gram stain: Purpose/ Introduction: The Gram stain reveals whether a microbe is gram positive or gram negative. It also reveals the shape and arrangement of the microbe (Professor Brady, Personal Communication). Of the forty-three possible microbes the following are gram positive: Bacillus cereus, Bacillus circulans, Bacillus coagulans, Bacillus megaterium, Bacillus subtilis,
- Little to no oxygen will result in the E. coli cells producing large quantities of acetic acid, causing the growth medium to reach pH 4 or lower however, with proper aeration the cells will be able to use many organic acids as carbon sources and the pH of the growth medium will be maintained at neutral or basic ranges. Aeration is another important factor in determining E. coli cell growth however it can continue to grow in the absence of oxygen using fermentation and anaerobic respiration. - Optimal growth temperature is 37C, cannot grow well at temperatures higher than 42C and they can tolerate lower temperatures with lower growth rate. - The doubling time or generation time for most E. coli strain in a rich medium at 37C is 20 minutes.
Major unknown #202 was given out by the instructor, and the unknown bacterium was streaked out on a Trypticase Soy Agar tube and plate to inoculating the bacterium and incubating. After incubated and grown the morphology was observed and several Gram stains were performed to determinate if the bacterium were gram positive or negative, and the morphology of the bacterium. The Gram Stain of my major unknown #202 was determinate to be Gram negative bacilli, and was double checked by the Gram check slide. Also I noticed that my bacterium was a facultative anaerobe and according to my results of endospore test, my bacterium has not endospores. So according to the list of possible major unknowns provided by the instructor, I narrow my bacterium thru
INTRODUCTION Clostridium perfringens , from the Clostridiaceae family , is a large non-motile Gram-positive bacteria . According to Johnson E. A., et al, Clostridium perfringens are “spore forming bacteria (subterminal spores) that are encapsulated in tissue smears” (2007). It has 5 different types of strains from strain A to E which each produces different toxins. Strain A and C are both pathogenic for humans. This bacteria are able to produce energy via anaerobic respiration thus does not produce colonies on agar plates which continuously exposed to air and commonly the cause for food poisoning which usually caused by Clostridium perfringens enterotoxin (CPE).