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Camel Milk

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Camel milk (Camelus dromedaries) taken from Rajasthan was serially diluted and spread on MRS agar plates and were incubated for overnight at temperature of 34°C. After incubation, we isolated the 24 individual colonies which were also stored for long term preservation by preparing the glycerol freeze stocks. Therefore, for further investigation these isolated samples for their probiotic potential were checked on the basis of their antimicrobial activity against the bacterial strains, Escherichia coli, Staphylococcus epidermidis and Bacillus subtilis.
3.2 PROBIOTIC PROPERTIES
3.2.1 Antimicrobial Activity
To study the antimicrobial activity of isolated colonies from camel milk. We were able to select 8 isolates out of 24 which have shown resistance …show more content…

TOLERANCE TO BILE CONCENTRATIONS

TABLE 3.2.3

Strains 0% 0.10% 0.20% 0.30% 0.40% 0.50%
01 0.628 0.508 0.175 -0.0175 -0.094 -0.061
02 0.708 0.349 0.149 -0.0375 -0.101 -0.043
07 0.627 0.187 0.459 -0.0065 -0.093 -0.063
08 0.733 0.151 -0.016 -0.0135 -0.0845 -0.066
11 0.641 0.147 -0.017 -0.0165 -0.0965 -0.0505
14 0.609 0.446 0.065 -0.0380 -0.078 -0.0435
15 0.656 0.396 0.103 -0.0385 -0.092 -0.0380
16 0.651 0.303 0.029 -0.0090 -0.098 -0.0415

Strains Vancomycin ZOI(cm) Oxacillin ZOI(cm) Clindamycin ZOI(cm) Kanamycin ZOI(cm) 01 v 4 3 02 1.8 3.2 3.5 1.5 07 2.1 3.8 3.5 1.8 08 -- 3.7 -- 1.0 11 -- 4.5 4 14 -- 3 3.5 15 v 4 4 16 -- -- 4

ANTIBIOTIC …show more content…

Strains Morphology Catalase Motility Gram Staining Methyl Red Arginine Production Acetoin Production
01 Cocci - - + - + -
02 Cocci - - + - + -
07 Cocci - - + - + -
08 Cocci - - + - + -
11 Cocci - - + - + -
14 Cocci + - + - + -
15 Cocci - - + - + -
16 Cocci - - + - + -

PHYSIOLOGICAL AND BIOCHEMICAL

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