Capecitabine And Gu Case Study

Capecitabine and Gum kondagogu (GK) (Grade-1) was purchased from Girijan Cooperative Corporation, Andhra Pradesh, India and Capecitabine was a gift samples by Dr. Reddy’s Research Foundation, (Hyderabad, India). Hydrochloroauric acid (HAuCl4) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Monochloroaceticacid acid was purchased from Hi-media Lab. Pvt. Ltd. (Mumbai. India). Methanol, sodium hydroxide and glacial acetic acid were purchased from Merck Limited (Mumbai, India).
Human cervical carcinoma cell line (HeLa) was obtained from National Center for Cell Science (NCCS) (Pune, India). 3-(4,5-dimethylthiazool-2-yl)-2,5-diphynyl tetrazolium bromide (MTT) and Dulbecco’s PBS (pH 7.4) were purchased from HiMedia Lab. Pvt. Ltd. (Mumbai,

The capecitabine solution was added to GNPs dispersal and stirred at 1000 rpm for 30 min. The mixture of capecitabine and GNPs dispersal was incubated for 24 h at room temperature for complete loading of drug on NPs and the centrifuged at 20,000 rpm for 30 mins. The obtained pellet after centrifugation was separated from the supernatant solution and redispersed in deionized water prior to further characterization. The capecitabine concentration in redispersed pellet was determined spectrophotometrically at the λmax value of 240 nm, the percentage loading of capecitabine on GNPs was estimated by following

The size of the GNPs and drug loaded NPs were done on TECHNAI G2 F30 S-TWIN instrument (FEI Company, Hillsboro, OR, USA) operated at an accelerated voltage of 200 kV with a lattice resolution of 0.14 nm and point image resolution of 0.20 nm. The surface charge and zeta potential of GNPs before and after loading of drug was determined by using the dynamic light scattering (Malvern instrument Ltd, Malvern, UK) as such without dilution. FTIR of spectra of CMGK, CMGK capped GNPs, native drug and drug loaded GNPs were recorded in KBr pellets using FTIR instrument IR Affinity-1 (Shimadzu) in the scanning range of 400-4000