Potato Enzyme Lab Report

We started of by adding a small piece of liver to an empty test tube and placing just enough water to cover it. After this we placed it in a hot bath for five minutes and after letting it cool we tested to see its reaction rate with hydrogen peroxide, it received a one because of the very little reaction occurring. Then we got four more empty test tubes and filled two of them with hydrogen peroxide and two of them with equal amounts of liver. Then we placed a test tube with hydrogen and another test tube with liver into an ice bath and placed the remaining into a warm bath for three minutes. After this we tested the reaction rates by pouring the respective hydrogen peroxide and live (warm with warm and cold with cold).

Purpose “What was the purpose for this lab?”- IRP Instructions The purpose for the Catalase Lab Experiment to have figured out if Hydrogen Peroxide could be broken down by catalyst found in and not in a living system. Background Information Biological catalyst have enzymes which contains

In this experiment, the evolution of the copper cycle was observed through a series of reactions. Four different copper compounds are formed through different reactions to inevitably lead to the recovery of Cu(s). This primary goal of this experiment was to study the Law of Conservation of Mass and perform 5 reactions on copper compounds. As Jenna Winterberg states in her book “Conservation of Mass,” the first part of this law is that mass or matter cannot be created. The second part of the law is that mass or matter cannot be destroyed .

Joshua Miller 12/18/17 Fermentation Lab report Introduction The term fermentation refers to the chemical breakdown of a substance by bacteria, yeasts, or other microorganisms, typically involving effervescence and the giving off of heat (wikipedia). Sugars are converted to ethyl alcohol when fermentation happens. In this experiment we determined if yeast cells undergo fermentation when placed in a closed flask with no oxygen. Glucose and yeast are mixed together in a closed flask and allowed to incubate for about one hour. Then, tests are performed to determine if the products of aerobic and anaerobic respiration are present in the flasks.The citric acid cycle consists of a series of chemical reactions used by all aerobic organisms to release stored energy through the oxidation of acetyl-CoA derived from carbohydrates, fats, and proteins into carbon dioxide and chemical energy in the form of ATP (Biology).

Once a prevalent color change had been observed at approximately 4 minutes (blue green color) the crucible was set on the counter using the tongs to cool for approximately 5 minutes. The appearance after this period resulted in another color change back to white. The crucible, lid, and hydrated copper sulfate was weighed again to calculate the mass of water lost by dehydration (described in table 1.3). This was done by subtracting the final mass by the initial mass of the crucible, lid, and compound. The mass of the crucible would remain unchanged while the mass of the compound would be altered.

1 “substrate” and another “ enzyme.” Instead of using the distilled water, this time you are going to use different pH buffer in the enzyme test tube. In the substrate tube, add 7 mL of distilled water, 0.3 mL of hydrogen peroxide, and 0.2 mL of guaiacol for a total volume of 7.5 mL. For the enzyme tube, instead of distilled water add the pH solution (3) and 1.5 mL of peroxidase which equals a total volume of 7.5 mL. Use the dH2O syringe for our pH solution. To clean the syringe, flush it by drawing 6 mL of distilled water.

This was repeated until no more gas was released. Next, the funnel was suspended through a ring, and 10 ml of 5% sodium hydroxide was added. When the two layers were separated in the separatory funnel, the aqueous layer was identified. The two layers were then separated into two different beakers. The water layer was acidified by adding concentrated hydrochloric

Fifty ml of salt effected the egg float the most, with average height 4 cm. Zero ml of salt effected the egg float the least, with average hight zero cm. Egg floated 0.3 cm in 15 cm of salt. Different amount on salt did effect the hight the egg floats in water. There are no obvious trends or patterns.

PARLE WAFFERS & FULLTOSS Potatoes used for making chips are stored in separate room (temperature less than the normal temperature). In that room, unwashed and unpeeled potatoes are manually put in the machine, from where they move to Destoner unit. PROCESS LAYOUT: 1. Destoner unit- wash potatoes in water. 2.

This reaction occurs through both oxidation and reduction. Oxidation is the process of a compound losing electrons by binding with oxygen. Reduction occurs when a separate compound accepts these electrons. In this particular experiment, the enzyme peroxidase, which is specified to break down hydrogen peroxide, will be used to catalyze the redox reaction. The substrates will be reduced guaiacol and hydrogen peroxide (H2O2).

The absorbance was read at 0 seconds, at 30 seconds, at 60 seconds, at 90 seconds and at 120 seconds. All the absorbances were remained 0 for the blank. After 120 seconds, the blank was then removed, and the appropriate amount of enzyme Tyrosinase (0.40 mL) was measured and added into the blank (cuvette #1) using the micropipette P-1000 according to the table 2. The final volume in the cuvette was 3mL. The cuvette contained the enzyme sample was wiped off with a KimWipe and was placed into the sample compartment of the machine.

After measuring the amount of water needed (50 mL), put the beaker of water back into the freezer to maintain its temperature. Pour 50 mL of cold water into one detached chamber. Place the chamber with water and the chamber without water on a table side by side. Place the connected choice chambers with 5 sowbugs in the right and left* chambers on top of the separate chambers (one with 10 degree celsius water and one without water), adjust the pairs of chambers so the attached chambers are directly on top of the separated chambers. Every 30 seconds count and write the amount sowbugs in each chamber (from 30 seconds to five minutes).

Four randomly selected Daphnia magna, for each trial, were removed from the provided colony for the bioactive compounds to be tested, and were transferred with a plastic wide-mouth pipette with approximately 10 mL of pond water to protect and ensure survival of the Daphnia. In order to acclimatize the Daphnia to laboratory conditions, they were then placed onto a petri dish on the Daphnia cooling chamber. The cooling chamber was located on the stereomicroscope platform and brought down the heart rate of the Daphnia to a range that was countable by the observer, since Daphnia heart rate at room temperature is too rapid. On the cooling chamber there were two petri dishes: one for the Daphnia that were going to be tested, and one with the Daphnia being tested on, to ensure constant consistent temperatures for each trial. To maintain a temperature conducive to the heart

While swirling the phosphoric acid solution in the Erlenmeyer flask, the sodium hydroxide solution was added to it a few drops at a time using a disposable plastic pipette. The After all the sodium hydroxide was transferred, the flask was rinsed with 2 mL of deionized water and added to the flask with the reaction mixture and swirled for an additional minute. A clean, dry evaporating dish with a watch glass was then weighed and recorded to 0.001 g. The reaction mixture was then transferred to the evaporating dish. The flask was then rinsed with 2 mL of deionized water and added to the evaporating dish containing the reaction mixture.