12. The TLC data obtained is provided in a table below. The TLC data was conducted solely in a 9:1 hexane/ethyl acetate solvent solution as opposed to the 1:1 and pure hexane solution as well. This was due to the lack of time, but as explained in number 7, a very polar solvent (1:1 solution) or non-polar solvent (pure hexane) is not ideal when obtaining
Abstract In this experiment, the isolation, characterization, and determination of concentration and purity of deoxyribonucleic acid or DNA from Allium Cepa or onion was performed. DNA was isolated through the use of a homogenizing solution. The absorbance ratio was 1.5, which indicates protein contamination. Moreover, the characterization of its components was conducted through the use of different chemical tests. The DNA gathered by the group bore positive results only on Test for Deoxyribose; compared to the standard solution, which bore positive results on all chemical tests, namely, Test for Deoxyribose, Test for Phosphate, Test for Purines, and test for Pyrimidines.
ANALYSIS REDUCING SUGARS From the observations obtained within the experiment, it can be concluded that only whipping cream and corn syrup tested positive for reducing sugars. However, it was hypothesized that in addition to whipping cream and corn syrup, the flour solution would also contain reducing sugars. This did not prove to be accurate as when it was tested with the Benedict 's solution, no evident change was recorded. Further research was conducted in order to determine why certain substances tested positive and the reason why others did not undergo any changes. To begin, during the Benedict 's and heat test, the test tube containing whipping cream underwent a colour change from a white, low viscous liquid to a bright orange hue
Throughout the experiment, there was a struggle to keep the heat stable which led to inaccurate data. Additionally, while changing the receiver from cyclohexane to toluene there was a loss of distillate which also led to the errors observed in the data. Furthermore, if more data were collected for each compound it would be a better representation of the experiment's results. If these errors were avoided, then the experiment would be more efficient in distilling the two compounds from each other and the plateau would be as sharp as figure 6 in the lab
This is only possible because aspirin decomposes to salicylic acid in a 1:1 ratio. ln[aspirin] was also calculated so that the plot obtained will show a linear relationship with time and allows us to analyse trends between the two variables. Although the data is a little sporadic, there is a visible trend of a gradual increase of salicylic acid concentrations and a decrease in aspirin concentrations. Fig 4. Plot of ln[Aspirin] against time The plot in Fig.
During the flame test, the cation was able to be identified by the color of the flame it produced from my previous tests results I was able to conclude that unknown solution A’s cation was calcium and unknown solution B’s cation was copper. During the precipitate test, halide, the anion wasn’t able to be identified but we do know that it is a halide ion. From previous results we can assume that unknown solution A’s anion is chloride and unknown solution B’s anion is sulphate. During the precipitate test, sulphate, the anion was able to be identified by the type of chemical reaction it produced from my previous results I can conclude that unknown solution A’s anion was calcium and unknown solution B’s anion was
Coconut oil in its true form is a solid at room temperature. However, if the lauric acid is removed, which is the case in MCT oils, the coconut oil will remain a liquid even at lower temperatures. Additionally, you will not receive the same health benefits as you would by using unprocessed coconut oil. Again, that doesn 't mean it 's bad for you, because using coconut oil in any form is still better than using any other type of
Substrate concentration basically means the amount used for the substrate. The substrate in our experiment was 0.1% hydrogen peroxide. The 0.1% is the concentration amount. Just like temperature and pH, substrate concentration can speed the reaction only up to a certain limit. When we mixed pH 3 enzyme tube with substrate tube, we used 0.3 mL of hydrogen peroxide, but if we were to increase the amount, then the experiment would have been faster.
Once those are removed, the water will now be safe for human consumption. One way to improve this experiment would be to collect the supernatant with a 0.50 mL syringe instead a 1.0 mL syringe. This way 0.50 mL of supernatant would be collected more accurately. It is a lot easier to collect the wrong amount when using a 1.0 mL syringe. Another way to improve this experiment would be to allow the fermentation reaction to happen for two weeks instead of one week.
The results were expected, since a difference in the odors of the enantiomers was detected, and the polarimeter showed that the two enantiomers rotated light to about the same degree in opposite directions. In the polarimetry experiment, the two absolute values of degrees of rotation were not exactly identical due to small errors caused by dust or other impurities in the sample. In the oil wafting experiment, some may not have been able to differentiate between the two enantiomers if their noses lacked the chiral receptors required to recognize this
During plating, drops of the Excedrin solution may have been introduced to the plate. Also, the capillary tube may have been contaminated. This lead to nearly identical marking on the TLC plate and similar Rf values. Due to this, it is not possible to conclude whether or not acetaminophen was separated from the sample of Excedrin. The Rf value of the pure isolated caffeine was 0.28.
When doing the experiment, some of the mass would be loss at some points. We can improved the percent recoveries by using more solvents to remove the crystals from the flask as much as possible. Its possible that small substances of the crystals were still in the flask. 3. Were the recrystallization effective (did purification result)?
The committee felt that quetiapine, although not having randomized controlled trial data to support its use, did have the data to support its safety, whereas all other atypical antipsychotics, excepting clozapine, did not. Aripiprazole is to be consider the third line agent, although data indicate that it is likely to worsen some patients. Cholinesterase inhibitors have also been used but not reported nearly as extensively as the AA. The data suggest mild benefit only, and the benefit may take weeks to develop. Rare case reports of electroconvulsive therapy (ECT) as an effective treatment for psychosis have been published.
When determining the solubility of malonic acid in different solvents both water and methyl alcohol were found to be polar when mixed with malonic acid. Hexane however was insoluble. Lastly biphenyl was mixed with water and was found to be insoluble, methyl alcohol was determined to be partially soluble.Hexane on the other hand was the only soluble solvent for biphenyl 2. Part B. of this experiment determined the solubility of different alcohols in hexane or water. Alcohols 1-Octanol, and 1-butanol were both found to be soluble in hexane while methyl alcohol was determined to be insoluble.
From the separation mixture, the aqueous layer were obtained and labeled as TT-1 (base), TT-2(acid) and TT-3 (neutral) in three different test tubes for later recovery. The procedure steps were followed as indicated which led to the correct identification of the tree compounds. The experimental melting points of acid and base were fairly close to the theoretical melting point. However the melting points of neutral were not as close due to some impurities in the compound. Determination of melting point for sample #124 acid and base were accurate due to no presence of impurities.