Empagliflozin Lab Report
DEVELOPMENT AND VALIDATION OF ZERO AND FIRST ORDER SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF IN EMPAGLIFLOZIN BULK AND PHARMACEUTICAL DOSAGE FORM N.PADMAJA2, G.VEERABHADRAM1 *
Department of Chemistry, University College of Science, Osmaina University, Hyderabad, India.
Faculty of Pharmacy, University College of Technology, Osmaina University, Hyderabad, India.
Corresponding Author Email: gvbhadram@gmail.com
ABSTRACT
Objective: Two simple accurate and precise, linear zero and first order Spectrophotometry methods were developed and validated for the estimation of Empagliflozin in bulk and pharmaceutical dosage form.
Methods: The estimation of Empagliflozin was carried out by using the zero order and first order derivative values and measured at 275 nm and 282 nm correspondingly. The estimation of the Empagliflozin was carried out by regression equations with the standard solution, sample solution.
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Precision i.e. intra-day relative standard deviation and inter-day relative standard deviation values was found be < 2.0%, accuracy was considered to be 98.89-101.32% .The LOD and LOQ value was found to be 0.1217μg/ml and 0.3690μg/ml for zero order derivative and 0.7526 μg/ml and 2.2807 μg/ml for the first order derivative correspondingly .No obstacle was observed from common pharmaceutical adjutants.
Conclusion: The method developed and validated can be utilized in its regular analysis Empagliflozin in quality control division.
Keywords: Empagliflozin, SGLT-2 inhibitor, Zero order spectra, first order spectra, UV-Spectro photometer.
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