The total run time was 25.0 min. Regression equations revealed good linear relationships for Pt and Pz (correlation coefficients: 0.9986 and 0.9989 respectively). This assay offers advantages in terms of practicality and suitability for the analysis of phloretin and phloridzin with acceptable validation results such as linearity, sensitivity, and recovery in terms of RSD (%).The method was linear in the concentration range of 50–500 ng/mL. The limit of quantification (LOQ) and limit of detection (LOD) was 68.74 ng and 20.62 ng for compound 1(PT) and 61.89 ng and 18.57 ng for compound
Pharmacokinetics of Tamoxifen (Nolvadex) of Tamoxifen was investigated following an oral dose of 20 mg tablet in eight healthy female volunteers. Plasma samples were collected at different time intervals following drug administration, and analyzed for Tamoxifen concentration by HPLC method. The mean ± SE (mean ± standard deviation) of Tamoxifen at 0.5 hour was calculated as 4.87 ± 0.82 ng/ml which shows drugs penetration in the blood immediately after oral dose. The minimum effective concentration of Tamoxifen is 30 ng/ml, that is 40ng/mL in another study (Santana et al., 2008). With the passage of time it reached at normal concentration at 2 hours 7.50 ± 0.13 ng/ml and then declined and at last became 26.1 ± 0.15 ng/ml at eight hours.
The following parameters were used: nano-ESI capillary voltage, 3.3 KV; sample cone, 35 V; extraction cone, 4 V; transfer CE, 4 V; trap gas flow, (2 ml/minute); IMS gas (N2) flow, (90 ml/minute). To perform the mobility separation, the IMS T-Wave™ pulse height was set to 40 V during transmission and the IMS T-Wave™ velocity was set to 800 m/s. The traveling wave height was ramped over 100% of the IMS cycle between 8 and 20 V. The time of flight analyzer (TOF) was calibrated with a solution of 500 fmole/μl of human [Glu1]-Fibrinopeptide B (Sigma-Aldrich), and the lock mass acquisition was performed every 30 s by the same peptide delivered through the reference sprayer of the nano-LockSpray source at a flow rate of 500 nl/minute. This calibration set the analyzer to detect ions in the range of 50–2000 m/z.The mass spectrometer was operated in the “resolution mode” with a resolving power of 18,000 FWHM, and the data acquisition was performed in “continuum” format. The data was acquired by rapidly alternating between two functions: Function-1 (low energy) and Function-2 (high energy).
The % RSD values were calculated. Interday precision: The Interday precision this method was checked by injecting six different preparations of proposed sample weight (100%) on next day of method precision. The % RSD values were calculated. Accuracy: Reliability and accuracy of the methods were studied based on recovery studies. Analysis was carried in three replicates with the spiking standard ascorbic acid (99.9% purity) concentration of 0.02 mg/ml at the levels of 50%, 100%, and 150%.
ABSTRACT A force degradation profile of Metformin Hcl & Glimepiride in combine tablet dosage form on RP-HPLC was developed using Grace RP-C18 (4.6 x 150 mm, 5 μm) in an gradient mode with mobile phase comprising of Acetonitrile: Dihydrogen Pott.Phosphate (pH 2.5 using 0.1% OPA) The flow rate was 0.7 mL/ min and effluent was monitored at 242 nm.. The retention times were found to be 2.06 min for MET and 5.80 min for GLIM. The assay shows a linear dynamic range of 250- 1250 μg/mL for MET and 1.0-5.0 μg/mL for GLIM. The calibration curves were linear (r2 = 0.999 for MET and r2 = 0.998 for GLIM) over the entire linear range. Mean % recovery was found to be 99.80 % for MET and 98.93 % for GLIM with % RSD was NMT 2 for both estimations which fully agrees with system suitability which is in good agreement with labeled amount of formulation.
for the determination of the minimum inhibitory concentration (MIC) of the active extract. The sStarting solutions of the tested extract were obtained by dissolving it in 5% dimethyl-sulphoxide. STwo fold serial dilutions twofold dilutions of the extract were made within a concentration range from 0.04 to 40 mg/mL in sterile 96-well plates containing Mueller–Hinton broth for bacterial cultures and a Sabouraud Dextrose SD broth for fungal cultures. Resazurin solution was added as an indicator to each well. and finally, to each well fungal or bacterial suspension was added .
No. Parameters VALSARTAN NIFEDIPINE 1 Linearity Range (μg/ml) 4-20 1.5-7.5 2 Regression Line Equation (y = mx + c) y = 39992x+68009 y = 11484x-18115 3 Correlation Coefficient (R²) 0.999 0.998 4 Intraday Precision (%RSD, n=3) 0.3584 – 0.5033 0.2983 – 0.4804 5 Interday Precision (% RSD, n=3) 0.4214 – 0.6017 0.4378 – 0.5492 6 Repeatability (% RSD, n=6) 0.5635 0.4628 7 LOD (μg/ml) 0.1067 0.2078 8 LOQ (μg/ml) 0.3236 0.6298 9 % Recovery Study (n=3) 99.50 – 100.15 99.33 – 100.40 REFERENCES: 1. Indian Pharmacopeia 2010, Ghaziabad: Govt. of India Ministry of Health and Family Welfare, The Controller of Publication Indian Pharmacopoeia Commission, 2010 vol- 1, 3, pp 382, 456, 1779-1780, 2287. 2.
The values of K for solutions 1-5 and U were 4.39E4, 4.53E4, 4.23E4, 4.70E4, 6.35E4, and 4.03E4 respectively. The average K for the lab was found to be 4.71E4 and the standard deviation was 8.302E3. The range then that all experimental values of K must fall under is 3.05E4 to 6.37E4. All experimental values of K for this trial fell within the range. Therefore, K can be determined a true constant.
It is available in local pharmacies as capsules for oral administration [Each capsule containing 300mg of gemfibrozil. Several HPLC methods in various body fluids[4-10] and one UV-spectrophotometric method in pharmaceutical formulations have been reported and published for gemfibrozil assay. This fact prompted the author to develop a simple, inexpensive UV-spectrophotometric method for the determination of gemfibrozil in pure and in dosage forms. The present research paper describes the development and validation of the UV-spectrophotometric method for the assay of gemfibrozil in pure and from its formulation (tablets) as per ICH validation guidelines using double distilled water as
5.1 MATERIAL USED Azathioprine was obtained as a gift sample from M/s Psycorem Pharmaceuticals Ltd. (Ludhiana, India); Carbopol 934 was purchased from Sunchem (India), Tween 20 and Span 20, Methyl and Propyl paraben, Light liquid paraffin, Propylene glycol, Triethanolamine (TEA), and Ethyl alcohol were purchased from M/s Central Drug House Pvt. Ltd ( New Delhi, India). Doubled distilled water was used throughout the study. 5.2 METHOD OF PREPARATION OF AZATHIOPRINE EMULGEL 5.2.1 Preparation of Emulsion The oil phase of the emulsion was prepared by dissolving 0.9 mL of Span 20 in 5 ml of Light liquid paraffin while the aqueous phase was prepared by dissolving 0.6 ml of Tween 20 in 25 ml of purified water. 30 mg of methyl paraben and 10 mg of