Estimation of superoxide dismutase (SOD) activity 246 SOD activity was estimated by its ability to catalyse NBT to formazan at 560nm 247 according to the method of Beyer and Fridovich (40). Five ml of reaction mixture 248 containing 50 mm phosphate buffer (pH 7.8), 13 mm methionine, 75 mm NBT, 2 mm 249 riboflavin, 0.1 mm EDTA and the enzyme extract. Absorbance of sample was read at 560 250 nm. The difference of percentage reduction of colour development in blank and the 251 sample was calculated. Fifty percent reduction in the colour was taken as one unit of 252 enzyme activity and was expressed in enzyme units per milligram protein (U mg-1 253 protein).
The third value calculated was the moles of iron used, using the formula of grams of iron used, multiplied by 1 mole of iron over the gram atomic weight of iron. With values inputed, the formula was 2.4075*1 mole/55.85 grams, equaling .0431 moles of iron. The next value calculated was the moles of copper produced, figured out with the formula of moles of iron used multiplied by moles of copper over moles of iron used. With values inputed, the formula was .0431 moles of iron*1 mole of Cu/1 mole of iron = .0431 moles of copper. The fifth formula used for the purpose of calculating the grams of copper produced was moles of Cu multiplied by the gram atomic weight of copper over moles of copper.
Based on the stoichiometric, the theoretical yield, actual yield and percent yield can be determined. In order to calculate the percent yield, the value for theoretical yield is required. Percent yield is determined by diving the actual yield by theoretical yield and multiplying by 100. The theoretical. The theoretical yield for Zinc Sulfide is 0.49 grams but the actual yield is 0.38 grams.
Convert gallons to barrels. a. 8,638,146,989,472 gallons = 274,226,888,157.04 bbl 4. The total of barrels multiplied by the porosity which is 2.5% or .025. a. 274,226,888,157.04 barrels x .025 = 6,855,672,203.93 bbl of produced water.
For calculations, refer to Appendix E. Finally, multiplying Ymax by Avagadro’s number will give the number of AA molecules adsorbed on the surface of one gram of charcoal at saturation. Since the approximate surface area occupied by a single AA molecule is 2.1E-19 m2, one can calculate the total surface area of one gram of charcoal. For calculations, refer to Appendix F. Note that all uncertainties from several dilution steps, weighing of the charcoal, the titration steps, and every measurement are taken into account when determining Y and C/Y. The calculated uncertainties are used to draw the error bars on the plots in Figure 1 and Figure
The three petroleum ether extracts were combined and concentrate to its minimum volume by using a stream of nitrogen. Analysis of fatty acids methyl ester (FAMEs) was carried out by Gas Chromatography using( Hewlett Packard, Palo Alto, CA, USA) (HP 6890) and(FID) detector was used at 250 °C . The fatty acid methyl siloxane capillary column HP – 5 (30m x 0.32 mm I.D. × 0.25 μm film thickness) was used. Nitrogen was used as the carrier gas (0.8 m / min gas flow).
What is the molecular formula of this hydrate? *Answers to questions above... 1) 4.078 + 4.056 + 4.095 + 4.014 = 16.24/4 = 4.061 ±0.008 grams 2.253 + 2.256 + 2.261 + 2.249 = 9.019/4 = 2.255 ±0.012 grams 2) Mass of water = 4.061 – 2.255 = 1.806 ±0.020 grams Then convert to moles =1.806 ±0.020 / 18 g = 0.100 ±0.020 mol H2O Covert to moles of copper Sulfate =2.255 ±0.012 / 159.6 = 0.0141 ±0.012 mol CuSO4 Finally simplify those values by dividing by the smallest number =0.0141 ±0.012 / 0.0141 ±0.012 = 1 Then: 0.100 ±0.020 /0.0141 ±0.012 = 8 3) Molecular Formula= CuSO4 x 8H2O
Explain what is meant by relative atomic mass?- Relative atomic mass is the measure of the mass of an element which consists of the protons and neutrons; it is always the bigger number and has the symbol Ar. An obvious example of this is oxygen has the Ar of 16 yet sodium has the Ar of 23. Explain how relative formula mass is calculated using the periodic table- You need to find the relative atomic mass (M) for each atom but this can only be done when the relative atomic mass have been found, then you add all of the masses in the compound together to find the RFM. Sodium Hydroxide is the example we used within our experiment. Na= 23 O= 16 H= 1 NaOH 23 + 16 + 1 = 40 NaOH Mr= 40 Explain how it is possible to work out amounts of substance
AIM To design an investigation to study the kinetics of a reaction of your choice RESEARCH QUESTION With respect to hydrochloric acid (HCl), what is the order of reaction in the reaction between HCl and calcium carbonate (CaCO3) determined by changing the concentration of HCl and measuring the volume of carbon dioxide gas (CO2) collected in 30 seconds whilst keeping the mass of the powdered CaCO3 constant and the temperature of the reaction system at 25oC? BACKGROUND INFORMATION Calcium carbonate (CaCO3) is a chemical compound that is commonly found in rocks such as chalk, limestone, marble and travertine in all parts of the world. It also used as a form of medicine as a dietary supplement for a person with insufficient calcium intake because calcium is needed by the body for healthy bones, muscles, nervous system, and heart. CaCO3 is also used as an antacid to relieve
In order to do this the scientists will measure the volume of gas that is produced within a 10 second interval time after the tablet begins to react. Then the scientist will observe the different rates of reaction with temperature. The Boltzmann distribution of law, indicates that high temperature makes molecules gain high energy contents (pubs.acs.org/doi/abs/10.1021/ja). In order to measure the reaction rate, the scientists must use the same volume of water at three different starting temperatures: hot tap
- A hydrate is a salt that contains water as a part of its crystal structure. The hydrate used in this lab was Copper (ll) Sulfate Pentahydrate. To heat the hydrate in this lab a crucible is needed. A crucible is a heat resistant container used to heat things to high temperatures. In this lab a mole was used to determine the measurements of all substances.