The final volume was adjusted with the same solvent to get concentration of 100 µg/ml. the solution was further diluted to get the concentration of 10 µg/ml, filtered through 0.45 µm filter tips , and aliquots of 20 µl from this solution was injected into HPLC by using an
Details on the preparation of anhydrous MgCl2 have been described previously . 17.8 g anhydrous KMgCl3 and 10 g anhydrous MgCl2 (the molar ratio of KMgCl3:MgCl2 was 1:1) were maintained at room temperature and 450 oC in constant temperature and humidity chamber for different times, respectively. Then the products obtained were used for analysis. The hygroscopy at room temperature could be reflected by the weight increase of the sample, and the hygroscopy at 450 oC could be reflected by w(MgO)/w(MgCl2) of the sample. 2.4.
Another 5-mL test tube, labelled as B, was filled with 1 mL of distilled water. A drop of methyl red was added. Also, a 0.01M hydrochloric acid (HCl) was added in a dropwise manner from a syringe until the color of the solution matches that of the first test tube setup. The volume of the HCl used was recorded for the determination of the ionization constant of
The first step was to disperse 4 g of block P123 (EO20PO70EO20) in 40 ml of distilled water and 120ml of 2 M aqueous HCl was added with stirring at 350 C for about 3 h. To this solution, 4 g of TEOS was added and continuously stirred at 400 C for 24h. Then the resulting gel was
Material: To do this experiment some particular tools and instruments are needed. The Burette is used to put bace NaOH in it, it has an error of ± 0.1ml. In titration experiment the burette is hanged on holder above the Conical flask. The conical flask has the acid HCl in it, and it is the place where the acid react with the base. The Pipette is the instrument that used to measure the amount of the acid HCl before adding it to the conical flask, it’s error is ± 0.03ml.
Initially eye piece micrometer was calibrated by using stage micrometer. A drop of suspension of microspheres in liquid paraffin was taken on glass slide, which was observed under 45 X. Sizeof 100 particles were measured. Drug content and drug loading Microsphers equivalent to 100 mg of DLTZH/ND was taken, crushed and dissolved in methanol, made up to 100 ml with methanol in a volumetric flask. This solution was diluted suitably to Beers’ range of the drug. Absorbance of the solution was measured at 238 nm.
Soap will therefore be much more effective in soft water than in hard water. The steps for the saponification soap making method can therefore be simplified into four: • Saponification: The fat and oil is mixed with the alkali and heated. The soap produced is the salt of a long chain carboxylic acid. • Glycerine removal: Saturated salt solution is added to dissolve the glycerine in the wet soap. A greater part of the glycerine is removed and separated from the soap whiles the other part remains to smoothen and soften the soap.
(2) Titration of Acetic Acid with Sodium Hydroxide 10ml of distilled water was added into a 250ml Erlenmeyer flask. 20ml of diluted acetic acid was then added into it, followed by setting up a titration system with 0.100M of NaOH in buret. A pH meter was used to monitor the pH of the solution as the base (NaOH) was added. Sodium hydroxide was added in an increment of 1ml until the solution pH reached 4.8. After the solution pH reached, the base was added in an increment of 0.2ml until the equivalence point was passed.
10ml of the sample was added to the 0.05 ml of 0.5% phenolphthalein indicator. It was mixed and allowed to stand for few minutes and was neutralized with 0.1M NaOH to the standard pink colour. 2 ml of formalin was added, mixed and allowed to stand for few minutes. The new acidity produced was titrated against 0.1M NaOH for the same pink colour. Then 2ml of the formalin and 10ml of H2O were titrated separately against 0.17M NaOH as blank.