INTRODUCTION Lipidsaareaa group of naturally occurringamolecules that includeafats, waxes,asterols, fat-soluble vitamins (such as vitamins A, D, E, and K) monoglycerides , diglycerides ,triglycerides, phospholipids, and others. The main biological functions of lipids include storing energy, signaling, and acting as structuralacomponents of cellamembranes. Lipids have applications in the cosmeticaand food industries as well as in nanotechnology. (Ackman, 1967) The iodine value (or "iodine adsorption value" or "iodine number" or "iodine index") in chemistry is the mass of iodine in grams that is consumed by 100 grams of a chemical substance. Iodine numbers are used for determining the amount of unsaturation in fatty …show more content…
Hanusaiodine solution, chloroform, aqueous KI solution, Na2S2O3 and starch solution is used. Iodine values are calculated from the difference between the blankaand the test sample. For peroxide value; solvent mixture (composed of glacial acetic acid and chloroform), saturated KI solution, starch solution and Na2S2O3 soluiton is used and peroxideavalues are calculated. A) Iodine Value: Hanus Method In this experiment, iodine value of sun floweraoil was determined with Hanusamethod. Blank solution and oil solution were prepared and stored in the dark. Then, they were titrated with Na2S2O3 and volume of titrant was determined for blank and oil solution. Finally, iodine value was determined by using volumeaof titrant. B) Determination of Peroxide Value In this experiment, peroxide value of sunflower oil was tried to determine. After preparing a solvent mixture, it was titrated with sodium thiosulphate but during titration time color change was not observed. The experiment was not completed successfully. METHODS AND MATERIALS A) Iodine Value: Hanus …show more content…
Then, putting of choloform in amount of 10 mL and solution of Hanus iodine as amount of 10 mL into conical flask is realized. Addition of these two substance into otheraflask also occurs for blank. Next, waiting for these two samples for exactly 30 minutes is realized. Afterthat, solution of potassium iodine in amount of 15 mL and 40 mL water being distilled are added. Titration of last mixture is performed in company with 0.1 M Na2S2O3 until the obtaining of color in yellow. Next, addition of starch indicator occurs and then titration of mixture in company with Na2S2O3 is realized until observing of color as blue is obtained. All of these procedure is repeated for solution of blank without adding oil. Consequently, quantity of iodine being used for absorption by oil is
The hypothesis that was provided to this question was If Phenol Red is add with the other chemicals then a color change will occur. Methods: To begin the lab 40 mL of Phenol red were obtained in a beaker, and 40 mL of water were obtained in a separate beaker. Next one plastic baggie was
ABSTRACT To catalyze a reaction, an enzyme will grab on (bind) to one or more reactant molecules. In this experiment we examined how increasing the volume of the extract added to the reaction would affect the rate of the reaction. The enzyme used was horseradish peroxidase which helps catalyze hydrogen peroxide. Using different pH levels, the absorbance rate of the reaction was measured to see at which condition the enzyme worked best. The rates of absorption were calculated using a spectrophotometer in 20 second intervals up to 120 seconds.
Nonetheless, the light yellow solid was purified by using the recrystallization technique. The formation of o-nitroacetanilide is inevitable and in order to eliminate it, 95% ethanol is used as the solvent of choice. The ortho isomer is soluble in the cold alcohol solution whereas p-nitroacetanilide in insoluble. As a result, the ortho isomer remains in the liquid solution and the final product, the p-nitroacetanilide is isolated with a final vacuum
The enzyme of turnip peroxidase is added in the equation to catalyze the oxidation. Objectives The objective
Before adding the iodine solution, the initial reading of the burette was taken. Then, the titration was started using the iodine solution into the burette with continuous swirling of the flask slowly and carefully. Once the color change started to appear, titration was stopped and final burette reading was recorded. Finally, the amount of vitamin C in the mandarin orange was calculated by using the standardization factor and used iodine solution.
These color changes indicate a chemical change, which show that a reaction had occurred. In the first step when o-vanillin and p-toludine, imine was formed. The color change from green to orange suggests that imine appears as orange colored. In the second step, the addition of sodium borohydride reduced the imine into another derivative, which was yellowish lime color. The solution turned clear when acids and anhydrides was added, which indicated the precipitate were dissolved.
Iodine-131 Iodine-131 is a radioisotope used in the treatment of thyroid cancer and is considered one of the most successful types of cancer treatments. Iodine is stored within the thyroid gland of the body. Humans are unable to make iodine so it must be absorbed through food. Iodine is necessary for the thyroid gland to be able to produce hormones. Iodine deficiency can also be very dangerous and lead to many different health problems.
This liquid is mixed using large paddles to mix air into the mixture and oxidize it. When the indoxyl is oxidized, it turns into indigotin. Indigotin is denser than the rest of the liquid, so it settles to the bottom of the vat. This pigment is then sent to the third and lowest vat. To prevent the pigment from fermenting, it is heated in the vat.
The iodine test determines the presence of starch in biological materials. It is predicted that, if starch is not present, the solution with iodine remains yellow. However, if starch is present the solution with iodine becomes a blue-black colour. Plants have starch as the storage polysaccharide (glucose units held together by glycosidic bonds) while animals have the equivalent of glycogen. In this experiment, the dark blue colour is visible because of the helical amylose and amylopectin reacting with iodine (Travers et al., 2002).
In test tube E, a colourless colour formed. It is because redox reaction occurred during the test. Idoine reduced into idoine ion , which changre from brown to colourless. In test tube F, the iodine solution change from brown to purple . It is because the salt has a function of cofactor which will shorten the time for amylase to take to break down the
Properties of Substances Express Lab 1)The purpose of this lab was to compare the physical properties of different types of solids and how the properties of solids are determined by their intermolecular forces and their intramolecular bonds. Then we were to classify each type of solid as either ionic, metallic, non-polar molecular, polar molecular, or network. Paraffin wax classified as a non-polar molecular, Silicon dioxide was classifies as a network, Sodium chloride was classified as ionic, Sucrose was classified as polar molecular and Tin was classified as metallic. (2)The intermolecular forces that are present in Paraffin wax are dispersion forces, because it is non-polar and carries a negative charge. Followed by Sucrose that has
Through the titration process, we are able to identify physical changes to the mixture such as the colour change to indicate the end point of the experiment. For example, the colour changes of phenolphthalein from colourless to pink and methyl orange from red to orange and subsequently yellow. Acids produce hydrogen ions and bases produce hydroxide ions. This causes the indicator to change colour due to the colour difference from the undissociate molecules.
The solution with the pigments was spotted 15 times on both region A and region B and then allowed to dry. When the plate was dry it was placed into the tank for at least 20
It is often used in the selective identification of enteric bacteria including Salmonella and Shigella. The TSI agar has glucose, lactose and sucrose as the sources of carbohydrates. Phenol red is the acid base indicator incorporated in the medium. The TSI medium indicates whether the bacteria ferments glucose only, or lactose and sucrose with or without production of gas. Nitrate serves as a source of nitrogen for many bacteria.
This peculiar structural deviation prevents these Trans-fats from being processed by the human body. As you can imagine Trans-fats stay in the body for a long time and are absolutely difficult to remove. Other, notably ingredients like nitrosamine which are modified versions of the natural amino acids. Amino acids the building blocks and the smallest units of all proteins. They influence the liver to produce many harmful fats implicating in brain damage.