Malassezia sp. is the causative agent of dandruff, which is the most commercially exploited disease by the cosmetic industry. This study is aimed at the isolation of the different species of Malassezia from young adults in Coimbatore region. The predominant species isolated was M.symbodialis. Key Words: Malassezia, dandruff Introduction Dandruff and seborrheic dermatitis are common hyperproliferative scalp disorders associated with Malassezia globosa. Dandruff occurs in atleast 40- 50% of the general population and with a higher prevalence in tropical countries like India. Despite the global distribution, limited research has been conducted to date concerning the pathogenesis of Malassezia yeasts. The flakes on the scalp can be an embarrassing situation and is the most exploited disease by the cosmetic industry (Ranganathan and Mukhopadhyay, 2010). Its prevalence and severity is greatest in young men. Although a variety of antifungal agents are available in the market for the treatment of dandruff, yet complete control is far from the reach. The available products and drugs are unable to prevent recurrence. The daily use of many shampoos makes hair dull and the chemical contents have their own negative effects. Thus the present study is aimed to isolate and identify the …show more content…
In primary cultures, colonies are surrounded by an abundant precipitate, as in species of the M. sympodialis complex. Cells are spherical, 2.5–8 mm in diameter, and budding is monopolar on a narrow base. In contrast to M. furfur, this micromorphology is a stable character in M. globosa. Colonies of M. symbodialis were glistening, smooth, flat or with a slight central elevation. Growth of both the species occurred at all the different temperatures studied except M. globosa that was unable to grow at
Unknown Lab Report Abiola Oyewumi March 16, 2015 Unknown #16 Abstract An experiment was conducted to determine which of the following unknown bacteria was in test tube number 16: Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, and Salmonella typhimurium. Biochemical tests were used to help identify the unknown bacteria. The Citrate test, Urease test, Triple Sugar Iron Agar test, Voges-Proskauer test, and Methyl Red test were the biochemical tests used in this experiment.
“ Christian Gram was the one who tried to develop the procedure of Gram staining by attempting to create a way to differentiate stains of schizomycetes from the other tissue cells”(Bartholomew).The Gram-staining was used to determine the morphology of the bacteria by using the cell wall structure. When the bacteria is viewed under a microscope, morphology was seen. Bacteria can have one of the three basic morphologies. The bacteria was cocci, rod, or spirilla morphology. If the result of the bacteria is purple, the bacteria has a thick peptidoglycan layer.
Chapter 2 Activity #2 (1-10) 1. What is the English – language description for diagnosis code 696.1? Psoriasis 2. Describe the disease from question 1. Psoriasis- “is a skin disorder.
After incubation, a gram stain was performed one the colonies that were isolated. First, the organism was smeared onto a slide with a loop full of distilled water. The smear was heat fixed to provide the bacteria to stick to surface. Next, the staining started by using crystal violet for 60 seconds, rinsed with distilled water. Then iodine for 60 seconds, rinsed with
Researches agree that there isn’t enough proof that this is effective for male baldness and that “more work needs to be done to test formulations of JAK inhibitors specially made for the scalp to determine whether they can induce hair growth in humans.” (Christiano, 2015) Mice were given
Nutrafol Review - Is this a Real Deal for Hair Loss Treatment? Get through this Review! Many individuals experience a variety of conditions that impact their lives greatly. In recent time, the incidence of hair loss has reportedly increased in both men and women. This can be a traumatic experience.
To prepare the solutions a 70% ethanol solution was used to make 40%. This was calculated using the C1V1=C2V2 formula. A photo spectrometer was used to measure, in arbitrary units, the change in membrane permeability of the B. Vulgaris cells. To begin, the B. Vulgaris samples were put into vials containing the distilled water, 40% and 70% Ethanol solutions. As soon as the B. Vulgaris samples were added to the vials a time zero sample was taken from the vials.
Part A: Osmosis practical task Aim: To observe the effects of osmosis in rhubarb cells. Hypothesis: Water will be extracted out of the cells in the salt solution causing the cells to look different to the cells in the freshwater solution. Materials: Rhubarb Distilled water in a dropping bottle Salt solution in a dropping bottle Microscope, slides, and coverslips Forceps and razor blades or scalpel Paper Towel Method: Clean and dry a slide and coverslip.
The reporter chose the classic sweet almond peppermint cleansing conditioner by Wen and started a 7 day regimen to see what would happen with her normally flat and slightly oily fine hair. To simply put it, the results were everything that Wen claimed they would be. She followed the routin prescribed, choosing for her own hair chemistry to do it in the mornings during a shower. Within a couple of days her hair had changed as a basil level. It had more shine, more body, more bounce and apart from finding that her curls fell a little
The B. Vulgaris samples were approximately 1cm3. They were kept the same size to ensure accurate results. A control test was conducted in distilled water to obtain a result to compare. The ethanol treatments were 40% and 70%. To prepare the solutions a 70% ethanol solution was used to make 40%.
Introduction: This lab report outlines an experiment on the observation of mitosis in the cells of garlic root tips. Mitosis simply put is the division of a nucleus producing two daughter cells with the same number of chromosomes as the parent cell. Miotic cell division consists of five stages: Interphase, Prophase, Metaphase, Anaphase and Telophase. The purpose of this experimet was to identify and observe cells within each stage of mitosis using garlic root tip cells.
The organism that was successfully cultured from the first lab was use to streak into other plates. Samples from the previously cultured MAC and blood agar were streaked on two different sides of an EMB, SS, and MSA plate. Samples of bacteria were also used to inoculate Citrate and TSI media. The inoculation of TSI and Citrate media were as follows: The materials were gathered, which include the previously cultured media, an inoculation needle and the Citrate and TSI medium.
The sterile cotton swab was inserted in the S. epidermidis culture and twirled around to obtain a specimen. The entire plate was inoculate with the swab from top to bottom, to achieve a lawn of growth. The dry forceps was used to remove the antibiotic disk into the appropriate spot on the plate. This process was repeated for the all antibiotics with aseptic technique being used. The plate was incubate with lid up on the bookshelf at room temperature for 48 hours.
Vinegar Pick a vinegar with natural fixings, for example, apple juice vinegar or balsamic vinegar. Vinegar is a decent solution for bothersome dandruff since it incorporates dynamic potassium compounds that diminish the itchiness. Tenderly massage the vinegar onto your scalp or empty some of it into your last mug of water while washing your hair. Aloe
Sordaria fimicola is a microscopic fungal species that produces ordered tetrads. It is commonly used in classrooms because it lacks conidiospores, has a short generation time with matching genotype and phenotype, has known color genes that permit tetrad analysis, easily observable crossing over effects, and does not undergo spindle overlap. In S. fimicola, meiosis occurs in the ascus. The fungus is a haploid organism for the majority of its life. It only becomes diploid when mycelia of two unlike strains fuse.