I will test the experiment to observe the process of osmosis. The membrane of the egg will all that will be left once the egg has been soaked in vinegar for the significant amount of time. After viewing a video over the experiment and reading on the background and science of it, I will understand why everything happens the way it does. This chemistry experiment has many learning experiences behind it that I will
To start gram staining, I slightly pour crystal-violet all over the slide and let it sit for 30 seconds before wash it off with water. Next, I dye the Unknown with Gram’s iodine to create a complex only have on gram positive. The slide is rinsed by water after 30 seconds. Decolorization is the next step of the whole process. I let the alcohol flow on 45-degree angle slide within 15 seconds and wash it with water to remove colors on the surface.
Analytical importance: The value is the relative measure of the amount of fatty acids that have been liberated due to the hydrolysis of the glycerides as a result of action of moisture, temperature and/or lypolytic enzyme lipase. Apparatus: • 250ml conical flask Reagents: • Ethyl alcohol: 95% alcohol or rectified spirit neutral to phenolphthalein indicator. • Phenolphthalein indicator solution. • Standard aqueous potassium hydroxide or sodium hydroxide solution 0.1N or 0.5N. The solution should be stored in a brown glass bottle and colorless.
To clean the syringe, flush it by drawing 6 mL of distilled water. Step 2: Mix both test tubes , shake gently and time the reaction. Step 3: The same step as procedure 1, and step 3 which is to record the observed color step 4: use the palette/color chart to help you identify the observations you make. Safety precautions: Pull your hair back Safety eye goggles Closed toe
The iodine test determines the presence of starch in biological materials. It is predicted that, if starch is not present, the solution with iodine remains yellow. However, if starch is present the solution with iodine becomes a blue-black colour. Plants have starch as the storage polysaccharide (glucose units held together by glycosidic bonds) while animals have the equivalent of glycogen. In this experiment, the dark blue colour is visible because of the helical amylose and amylopectin reacting with iodine (Travers et al., 2002).
After cutting a block of the fungus, we placed it on the petri dished in the section that was appropriately marked for that specific strain. After the four sections were placed, the lid was placed on the petri dish, and then the dish was with tape to keep close, and wrapped in aluminum foil and placed into the correct bin to sit. After two weeks the petri dishes were ready to be observed. We used a toothpick to scrap lightly the top of the agar in the petri dishes, ensuring we were collecting from one of the dividing lines. With the spores on our toothpicks, we then smeared them onto a slide that was provided to us.
The experiment began by setting up the LabQuest and preparing a 2M solution of HCl and a 2M solution of NaOH. This was called “Part A”. Two general rules were noted throughout the experiment: add acid to water and pour stock solution into beaker before graduated cylinder. This prevented flash-boiling of the solution, chemical burns, and spills. To make the 2M HCl solution, 200mL deionized water was added to a 600mL beaker labelled “2M HCl” by using a graduated cylinder.
The last step was the formation of Cu(s). This step recovered Solid elemental copper. Al(s) wire was placed in the solution from the last step and 5 drops of HCl along with a stir bar was added to the beaker and this was stirred on the hot plate. Cu(s) precipitate formed on the wire and the solution turned from clear to cloudy until it eventually become a brownish red color. When the reaction was complete the Al(s) wire was scraped with the stirring rod to get off any residual Copper product.
The purpose of the lab is to acquire the percent composition of zinc and copper. The procedure included obtaining a post 1983 penny and washing it with soap and water. Using a triangular file, we made an X on the penny. Then, we cleaned the top and bottom of the penny with steel wool until it was shiny. We rinsed the penny in acetone and dried it with paper towel.
To start off, I can use a fact I have in my background knowledge—that disinfectants, cleaners, and bleaches all have a high pH (between 9 and 12)—to make sense of the dead bacteria and missing protozoans. Disinfectants, soaps, and cleaners are all used to kill off bacteria and common germs. Because pH9 is around the same pH as disinfectants, soaps, and cleaners, the dots we observed were most likely exactly what we thought them to be; dead bacteria. This all makes sense, because we know what is supposed to kill off bacteria has the same pH, so maybe anything with such a pH will kill off bacteria. Also, it makes sense that one result of this would be that the protozoans disappear, or even die.
If the color changes, then it is known that monosaccharides are present in the solution. Next, one will test for starches. Starch solution is then placed into the test tube at a quantity of 5 mL. 5 drops of Lugol’s Iodine solution is added to the test tube. If the color changes, then it is known that starches are present in the solution.