In the round-bottom flask (100 mL), we placed p-aminobenzoic acid (1.2 g) and ethanol (12 mL). We swirled the mixture until the solid dissolved completely. We used Pasteur pipet to add concentrated sulfuric acid (1.0 mL) to the flask. We added boiling stone and assembled the reflux. Then, we did reflux for 75 minutes.
* In the above RB, a calculated amount of 1.2 equivalent amount of PTSH was added during continous stirring. * To the RB a condenser was attached and it was put on refluxing for a time period of 18 hours at a temperature of 80 degrees in an oil bath. * Post refluxing, the condenser was removed and it was left undisturbed for 1 day and then cooled to a temperature of -20 degrees by keeping in the freezer for setting of hydrazone crystals. * Once the crystals were formed, the supernatant was decanted and the crystals were transferred to a beaker where they were washed with cold methanol and then dried. * These dried hydrazone crystals were used in the subsequent steps.
To the double digest tube was added 12 μL double digest master mix, and 1.9 μL sterile dH2O. To the single digest tube was added 11 μL single digest master mix, and 2.9 μL sterile dH2O. To the undigested tube was added 10 μL undigested master mix, and 3.9 μL sterile dH2O. Digests were then incubated at 37 °C for one hour and then stored at -20 °C for one week. After which the digestions were examined by gel electrophoresis.
The 1:1 hexane to ethyl acetate solvent resulted in the best separation because it not only showed extra spots that the other solvent mixtures did not have, but also the 4 spots were relatively dispersed with Rf values at 0.77, 0.56, 0.27, and 0.10 (Figure 2). Missing spots were also noted on the hexane only TLC plate. The orange eluent was ultimately chosen as our major product because it had significantly different TLC results than the 3 yellow eluents with the same Rf of 0.23 (Figure 3). The percent yield for this purification method was 248% and the extrapolated percent purified yield was around 135 %, which are both erroneously high. These high percent yield may be due to extra water weight or not fully evaporated
5-aminotetrazole monohydrate: In a 250 ml round-bottom flask equipped with a condenser for refluxing (90 °C) and a magnetic stirring bar, 5.00 g (5.95 mmol) dicyandiamide (three times crystallized), 7.47 g (11.9 mmol) sodium azide and 11.00 g (17.8 mmol) boric acid and 100 ml of water is added and allowed to reflux for 24 hours, after the completion of the reaction, until the solution pH to about 2 to 3 as hydrochloric acid 37% is added (about 12 ml) Then the reaction mixture was cooled in a refrigerator for 18 hours and the white crystals formed. The mixture was filtered and washed three times with 10 ml of water and and dried in 60 °C for 5 hours and finally 45.8 g of product by it will be obtained. 5-Aminotetrazol monohydrate: Yield:,
Materials and synthesis Poly (viny alcohol) (PVA) (99%, Mw. 1,15,000; Loba ) was dried in a hot air oven about 80 ºC at least 12 hours before to use. Poly(oxy-1,4-phenylenecarbonyl-1,4-phenylene) (Mw. 20,800; Sigma aldrich) (PEEK) pellets were dried at 100 ºC in hot air oven overnight before to the sulfonation process. Montmorillonite K10 (Na+MMT) (Himadia), Ion exchange resin (CDH), sulfuric acid (1.84 g cm-3, 98 wt%; Merck), Dimethyl sulfoxide (DMSO) and methanol were used as recieved.
Repeat the experiment. The cola drinks were titrated using the following method: Prepare the beverage in a 250ml volumetric flask. Use a funnel to facilitate the process. Place the beaker on a hot plate so that it boils and place a watch glass on top to prevent the carbon dioxide from the atmosphere getting dissolved in the cola. Once the cola starts to boil, continue to boil it for another 10 minutes so that the carbon dioxide is removed.
Absorbance versus Time Measurements: The absorbance was set to 0 Abs while the spectrometer was set to ʎmax (from Part A). In Part B, 1.00 ml of the solution was mixed with the Blue dye in the beaker and half-way covered with a cuvette. Concurrently, the Spectronic 20 was blanked with water. The processes detailed above were repeated, each at a time. The absorbance values were recorded for 11 minutes.
A: Yes, we need molar mass of sulfur, hydrogen, and chlorine. Then set the molar mass of copper and zinc to x and y. And use the mole ratio, which is 1:1 for two reactions to solve the unknown. 4. How many grams of zinc chloride could be formed from the reaction of 3.57g of zinc with excess HCl?
4 Procedure Preparation of Test Solution The sample was prepared by adding 100 mg of powdered in 10 mL of methanol, soaked for 15 min. Centrifugation was done and the filtrates or supernatants were used as the sample solution. Out of this filtrate 50 μL of samples were dissolved in 1 mL of toluene and were used as the sample solution. Preparation of the standard solutions Wedelolactone, Ecliptaalbasaponin-I and Ecliptaalbasaponin-II were used as marker compounds; these markers were dissolved in methanol at a concentration of 1 mg/ml. The extracts of Bhrungaraj prepared were shaken and sonicated in methanol at a concentration of 10 mg/ml.
The silver ion TLC was prepared through the following procedure: Silver nitrate was dissolved in 10 ml of distilled water. This aqueous solution of silver nitrate was absolutely mixed with 9 g of silica gel (10 ~ 40 μm particles). Then, a 10 × 5 cm TLC plate was coated with the above slurry and activated for 1 h at 90 °C before use. They were immediately transferred into a desiccator in dark for storage after cooling. 32 100 μL of afore-prepared sample solution and the mixed reference standard were diluted 100 times with ethyl acetate.
Then carefully measure 25ml of methanol and 25ml of ethyl acetate using a measuring beaker 12. Pour each solvent into its respective labelled mortar 13. Stir each sample for 10 minutes using a stirring rod 14. Leave solutions in the sun for 12 hours, this is to allow for the active ingredients bond with the solvent and form a solution and to allow some of the Methanol and Ethyl acetate to evaporate. 15.