Major unknown #202 was given out by the instructor, and the unknown bacterium was streaked out on a Trypticase Soy Agar tube and plate to inoculating the bacterium and incubating. After incubated and grown the morphology was observed and several Gram stains were performed to determinate if the bacterium were gram positive or negative, and the morphology of the bacterium. The Gram Stain of my major unknown #202 was determinate to be Gram negative bacilli, and was double checked by the Gram check slide. Also I noticed that my bacterium was a facultative anaerobe and according to my results of endospore test, my bacterium has not endospores. So according to the list of possible major unknowns provided by the instructor, I narrow my bacterium thru …show more content…
So the results were sulfur: positive, indole: Negative, and for medium: medium motility. And the results indicated that could be Klebsiella pneumoniae but also it could be Enterobacter aerogenes. To confirm the deduction of hat my bacterium could be Klebsiella pneumoniae I decided to do my fourth test. And the Urea Hydrolysis and if the results turn negative the bacterium will be Enterobacter aerogenes, or if it turns positive the bacteria will be Klebsiella pneumoniae. The results turns pink after 48 hours of incubation and this indicated that the Urea Hydrolysis it is positive (slow). As a result this test confirm that my bacterium is Klebsiella pneumoniae. Klebsiella pneumoniae is a Gram negative rod bacterium that can form a capsule. K. pneumoniae can be found in the GI tract in humans and it can cause nosocomial urinary tract infections and pneumonia also it can be cause death. K. pneumoniae can be contracted be direct contact. Washing hands before and after had a direct contact with a patient can avoid and decrease the spread of K.
In this experiment, we cultivated an unknown specimen containing two microorganisms. The purpose of this experiment was to use a variety of biochemical test previously learned in the lab to identify the unknown bacteria. The identification of unknown bacteria is a major part of microbiology. Microbiologist observe samples such as blood and sputum in the laboratory for the presence of microorganisms. Identifying unknown bacteria is extremely important in clinical settings because it helps physicians find treatment for infections.
Figure 3. Testing of transformed and mutant bacteria on minimal medium Growth was observed on the Transformed (Trsf) section and not on the Mutant (Mut)
These microorganisms are used to teach us how multicellular organisms came to be and how they can survive today. These small, microscopic organisms are so unique that the identification of them is paramount in the advancements of science. Knowing the chemical makeup, the shape, and the biochemical processes is important in identifying these organisms to understand how they survive and where. A number of tests can be ran on an unknown bacteria to determine their ideal
Genetic engineering is changing the DNA code to express different traits. A plasmid is a circular piece of DNA that contains important genetic information. Recombinant DNA is the product after inserting your desired genes. The genes we hoped to insert in the pGLO lab were the GFP gene and the ampicillin resistance gene. GFP was needed so that we would tell if the ampicillin resistance gene had been properly placed when the bacteria glowed under a UV light.
Staphylococcus epidermidis is an opportunistic pathogen, meaning that they can cause infection more frequently and more easily in persons’ with a weakened immune system such as, HIV patients (CDC, 2015). This organism is seen primarily in nosocomial infections, also known as, hospital-acquired infections (Bukhari, 2004). This means that the organisms favors the hospital environment and is easily passed to a person while they are in the hospital. Ways that a person can get a Staphylococcus epidermidis infection is through IV’s, more commonly in drug users, catheters, and artificial equipment (Bukhari, 2004). Skin-to-skin contact can also be a form of getting this type of infection.
Being able to identify unknown microbes from systematic testing is what makes the field of microbiology so important, especially in infectious disease control. Using the testing procedure laid out by the microbiology field we are able to identify unknown bacteria present in our everyday lives, and along the way learn a lot about their characteristics that separate them from other types of bacteria. Being able to do this is vital in order for us to understand why microbes are present in certain places, how they are able to grow and what restricts their growth, that way they can be combatted if necessary. These techniques for determining unknowns are also important for isolating and testing infectious disease microbes in order to prevent spreading. Another important aspect of being able to identify unknown microbes is the
The unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not
Klebsiella is a type of bacteria that can be found in a healthcare setting, causes a bacterial healthcare related infection which leads to pneumoniae, bloodstream infections, wound/surgical infections, urinary tract infections, and meningitis. It can be found in the human mouth, skin, intestines (where they don't cause a disease), but also from the feces of humans (4). Sick patients who are already receiving treatment for another condition are usually the ones who receive this infection, from breathing machines, vein catheter. Healthy people don't usually get klebsiella infections (4). Also, pneumoniae is ubiquitous to the ecological environment, which includes water, sewage, and soil (2).
Unknown #10 produced no identifiable macroscopic characteristics as a broth, so the first step was to Gram stain a loopful to determine the microscopic characteristics. Gram staining not only helped identify Unknown #10’s microscopic morphology but it also helped ensure the specimen was a pure culture—no other bacteria were visible when Unknown #10 was Gram stained and observed under the microscope. Unknown #10’s key microscopic morphology was that it was a very small, Gram negative bacillus. Though bacilli can possibly form endospores, no empty white centers were visible which suggested that Unknown #10 was not an endospore forming bacteria. No quick endospore stain was performed to validate this assumption since only one assigned organism was endospore forming and unlike Unknown #10, that organism was Gram positive.
Exercise 14: Unknown Identification Lab Report The purpose of the study was to identify the unknown bacterium using various biochemical tests in addition to using scientific methods in determining the outcome of the hypothesis. Each biochemical test will help determine the bacteria based on specific characteristics of each organism. I was giving unknown number 232. The first procedure that needed to be done after obtaining unknown bacterial mixture was to isolate the two bacteria in a pure culture using the streak plate method described in Microbiology Laboratory Manual Eight Edition. The material used was trypticase soy agar (TSA) plate, nutrient plate, starch agar, hydrogen peroxide, iodine reagent and microscope.
After a gram stain was done unknown #257 was identified as a gram positive organism because when observed under the microscope the organism appeared purple with cocci in clusters. The organism was also catalase positive which means that it produced enzyme catalase and bubbled when hydrogen peroxide was added to it. Three test were conducted based on the result of the gram staining procedure. Blood agar with a Novobiocin disk was chosen as well as DNase (DNA) and Mannitol Salts (MSA) agar. The Blood agar is a bright red, opaque plate and the streaking or the inoculation technique was a modified streaking for isolation with a heavy quadrant one.
Another way Klebsiella can be transmitted is by person-to-person contact. Symptoms of K. pneumonia are fever, cough with mucus, shortness of breath, and other flu like
If the urine lab results show that the urine is too concentrated, this could be an indication that the patients kidneys are not functioning properly. If your lab results showed your urine had a pH of 6, then that is a great sign because that is the average pH in healthy individuals. Although your pH was in what is considered a normal range, the turbid appearance and amber color suggests that you need to increase the amount of water that you are drinking daily. Your lab results showed that there was no protein in your urine. It is important that a patient would have no protein in the urine because then they would have something called proteinuria.
After experiment on microscope under oil immersion, I learned that my Unknown is gram positive. Under the lens, the bacteria appears in purple color. Its morphology is cocci arranged in cluster. However, during decolorizing process, I put too much alcohol to the crystal violet-iodine complex making the color overly removed. That led to the result of my gram positive has slightly redish