Genetic engineering is changing the DNA code to express different traits. A plasmid is a circular piece of DNA that contains important genetic information. Recombinant DNA is the product after inserting your desired genes. The genes we hoped to insert in the pGLO lab were the GFP gene and the ampicillin resistance gene. GFP was needed so that we would tell if the ampicillin resistance gene had been properly placed when the bacteria glowed under a UV light. The purpose of this lab was to perform a procedure known as genetic transformation which allowed us to genetically engineer E. Coli to be ampicillin resistance. Before the lab we expected that lysogeny broth and minus DNA will have growth but no glow. The lysogeny broth, ampicillin, and …show more content…
Meanwhile, on our LB/amp plus DNA petri dish we had a lawn of bacteria and the color was almost clear. Our results for this petri dish mean that the bacteria was not killed by the ampicillin. Next, in the LB/amp minus DNA petri dish we observed very minimal amounts of growth. These results mean that we correctly followed the procedures because this petri dish was not supposed to have growth due to the ampicillin. Our last petri dish was minus DNA and lysogeny broth, in this petri dish we saw 7 colonies of bacteria and there were a light yellow color. These results mean that we didn’t infect that petri dish with ampicillin from the pipette. Our expectations were both unsupported and confirmed. For example, our minus DNA and lysogeny broth had bacteria growth while our LB/amp/ara plus DNA dish had no growth and did not glow. Some problems that might have occurred in the lab is that we either put too much ampicillin in our LB/amp/ara dish or we could have had all plasma and no bacteria. Something that was difficult for me personally was the disposing of the pipette tips when switching from different substances and pipetting the right amount. These difficulties might have caused me to make mistakes on the amount needed to pipette or the type of substance. Overall, next time I would have made sure to pay closer attention to the pipetting portion of the procedure. Furthermore, we could extend this experiment by trying different kinds of
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chinesis. A construct of R751::Tn4351 (the physical map of R751::Tn4351 and restriction sites are shown in fig. 7) was selected for introduction into F. chinesis to discover if the introduction and insertion of the vector R751 and the transposition of T4351 into the F. chinesis chromosome by a triparental mating occurred. One parent was E. coli GJ342 which carried a helper plasmid, the second parent was E. coli HB101 which contained R751::Tn4351 and the third parent was the F. chinesis target strain. 189 colonies were isolated on LB agar plates which in passage in fresh media were able to grow in 200µgml-1 erythromycin.
The unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not
To limit the amount of errors or contamination in any procedure lab safety rules, gloves, and the aseptic technique was strictly enforced throughout the experiment. The first step to identifying the unknown bacterium was the Streak Plate Method. This method is used to isolate a pure culture from a mixed culture. Also, this method included streaking a tryptic soy agar (TSA) plate into four quadrants, and later incubating the plate for 24 hours.
In the laboratory, identification of an unknown bacterium is often necessary. In the lab, a random sample consisting of three different bacteria was selected. The sample contained one gram-positive, one gram-negative paracolon, and one gram-negative coliform. The purpose of the experiment is to identify each of the three species that the mixture contained. After receiving an unknown mixture, the sample was streaked for isolation onto TSA, blood agar, and MacConkey plates.
The Mut section is expected to have no growth as mutants require the amino acids leucine and valine to grow which is not provided in the minimal medium. Results Figure 2. Testing of mutant mixed with DNA, mutant bacteria and DNA on LB medium Growth was observed on the Transformed (Trsf) section and the Mutant (Mut) section but not on the DNA section. Due to human errors, the photo of our experiment was lost, but we have obtained similar results as from group1.1 and their photo is presented.
and we were excepting to find glucose and salt outside but not starch as the molecule is too big. Generally, the results proved to be reliable in some aspect, i.e. the expected results of glucose and salt found outside the tubing in the surrounding water, but none of the tubes
Introduction: Genetically modified organisms can be defined as organisms in which the DNA has been changed in a way that does not occur naturally by any reproduction procedure. The enviropig is just one of many organisms that they did experiments on to modify it to have specific (needed) outcomes. The reason for genetic modification is to be able to change a product or organism so that it deliver desirable traits. The enviropig was created to solve the problem of pigs not being able to absorb enough phosphorous from their diet, which then in its turn contributes to the larger factor of pollution.
The putrid smell of Escherichia coli is one that is immediately identifiable to the few lucky individuals who recognize its scent. It is also an aroma with which I became intimately sensitive to as I shuttled petri dishes of the bacterium in and out of an incubator. While my classmates shied away from the task of handling the pungent bacteria used in our recombinant DNA experiments, I took to the task eagerly, anything that would take me one step closer to my goal of researching. I had the opportunity to learn about lab techniques and cutting edge biology concepts the summer before my junior year, in an extracurricular biotechnology class at Northwestern University’s Center for Talent Development. The class, a three week crash course in the
Discussion PV92 Gel Electrophoresis Results: Through the usage of gel electrophoresis the correct allele for each sample was able to be determined. Lanes one through three in the gel,were the positive control lanes they contained the PCR cocktail and a known high-quality template for the PCR reaction. First lane contained the sample with the +/+ allele, which had two copies of the ALU repeat allele. The first lane had a band at about 941 base pairs.
The needle was inserted in the test tube, making sure not to touch the side of the test tube. Inoculate the media by going through the agar, as the needle is pulled out; drag the needle across the top of the agar. This technique was applied to both the TSI and Citrate
Biochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining.
If the broth turned a reddish color, the result was then positive. If there was no color change, then a small amount of zinc powder was added. If there was no color change, the result was also positive, but if there was a red coloration development after the zinc was added, the result was then negative. Both Unknown bacteria (16A and 16B) were positive for nitrate reduction. The tubes were then