Measuring dormancy rate of Solanum torvum seeds using Pre-soaking treatment versus Control.
Introduction:
In their paper “Seed Germination Enhancement in Solanum stramonifolium and Solanum torvum,” Nur Eva Hayati, Sutevee Sukprakarn, and Sunanta Juntakool (2005) asserts that dormancy of the Solanum family prohibits the commercial viability of the seed. The authors surmised that the seed dormancy, which a block to germination of the seeds, could be broken to enable a shorter germination period. They utilized different storage periods, soaking times and chemical washes to test and support this hypothesis. The paper concluded that the treatments which removed inhibitors from the seed coat were a viable solution to the decrease in germination time of the Solanum family. (Hayati et al., 2005) This experiment attempts to answer the question which is, does pre-soaking break dormancy faster than planting seeds without
…show more content…
Before planting, each group secured sixteen (16) seeds in a coffee filter, labeled the filter with the designated group number and allowed the seeds to soak in warm water for one (1) hour. During the wait time, a flat of thirty-two containers was filled to the top with soil. The soil was saturated with tap water and a quarter (1/4) inch of water was also put in the reservoir in the bottom of the flat. A stake was labeled with the correct group number and inserted in one of the containers at an angle to allow for the top to be secured. Each container had one (1) Solanum torvum seed planted at a depth of three (3) times the size of the seed. Sixteen (16) of the containers held pre-soaked seeds and a control group of sixteen. The number of seeds that germinated was counted at seven days and again at twelve days and that number was recorded in excel sheet. The spreadsheet includes columns for each group number along with the amount and percentages of Control and Pre-Soaked
Name: Avishak Deb Roy Partners: Leevell Penn, Varugh, Butler Bio 101 Lab Report #1 02.22.2018 Swimming speed of paramecium tetraurelia in different levels of treatment. Introduction Paramecia is a unicellular Protista which are naturally found in aquatic habitats. It is easily cultured in the laboratory. It is oblong shaped and covered with short hairy structure called cilia. Paramecia does not pose any health or ethical concerns and the population can be maintained if there is a food source such as Enterobacter (Biological Foundation 7).
Introduction- We did an experiment about different types of movements called kinesis and taxis. Kinesis is the change in the speed of movement or the rate of turning which is intensified by a stimulus (Meyer, 2006). Taxis is movement towards or away from a stimulus (Meyer, 2006). We did our experiment using Sowbugs.
Daphnia Magna are small crustaceans that are excellent for observing the effect of depressants on their nervous system. The purpose of this specific experiment is to observe the effect of ethanol, aspirin, and caffeine on daphnia’s heart rate. Not only do these chemicals affect heart rate, but they also affect the frequency of reproduction, number of eggs produced, and body structure. Pesticides similar to these chemicals can be released into the environment and daphnia re useful in monitoring toxicity levels. The agents being tested in this experiment can be administered to determine the effects of hormones, stimulants, antidepressants, and neurotransmitters.
The difference in this chemical and physical properties will aid in their separation. Processes like solubility, gravitational filtration and recrystallization will be used to separate the substances present in Panacetin. The melting and boiling point of the substances will help in concluding on which of these compounds will be presented at the end of experiment. Procedure and observation The Panacetin content was weighed approximately 3.0493g and transferred to the Erlenmeyer flask; 75ml of dichloromethane (CH¬2CL2) was added to the content. The dichloromethane (CH2Cl2) dissolved the sucrose, leaving the active unknown agent and aspirin behind.
On day one no seeds germinated. By day two, seeds in the control group, 15% and 25% experimental groups had germinated. On day two the experimental group with 25% concentration of miracle gro’ had the most seeds
Cell Respiration Lab Research Question What is the optimal temperature for germinating pea-seeds where the rate of respiration is the greatest? Background Information Cell Respiration refers to the biochemical process conducted by the cells of an organism that combines glucose and oxygen to produce energy in the form of ATP, along with two by-products, water and carbon dioxide. The equation representing this chemical reaction is shown below. C6H12O6 + 6 O2 6 CO2 + 6 H2O
I. Purpose: To experimentally determine the mass and the mole content of a measured sample. II. Materials: The materials used in this experiment a 50-mL beaker, 12 samples, a balance and paper towels. III.
Tn 4351 was originally isolated from bacteroides fragilis [30] . The transposon was successfully introduced into Cytophaga succinicans, Flavobacterium meningosepticum, Flexibacter canadiansis, Flexibacter strain SFI and Sporocytophaga myxococcoides by conjugation [25]. Tn 4351carries two antibiotic resistance gene. One of the codes for resistance to erythromycin and clindamycin which is expressed in bactroides but not in E.Coli. The other gene codes for resistance in tetracycline and is expressed in aerobically grpwn E. coli, but not in anaerobically grpwn E. coli or in bacteroides.
In this three-week long experiment conducted in the Bio 13 Lab, we were able to analyze a single nucleotide polymorphism (SNP) in our own genomic DNA and then determine our genotype at this specific SNP. In week one, we extracted genomic DNA from our cheek cells with swabs and prepared our DNA for PCR (Polymerase Chain Reaction) that would amplify the region with the intended SNP of interest. After one week and after the PCR was run outside of the lab section, the resulting PCR product was purified and treated with restriction enzyme Ahdl in order to prepare for the final analysis of our genotypes. In the third and final week of the project, we analyzed our PCR products by means of agarose gel electrophoresis. By the conclusion of the experiment, we had completed the analysis at the SNP of interest and determined our genotypes for this SNP.
A. Were the seeds in the “N” tube undergoing both photosynthesis and respiration, only photosynthesis, or only respiration? Use your results in Data Table 2 to support your answer. The seeds in test tube "N" underwent respiration. Respiration is always happening.
Unknown Lab Report Mikee Lianne Gonzales Biol 351- 1005 Holly Martin Unknown: # 76 Abstract This report is about identifying the respective genus of the given unknown organism. The goal is to show and prove the student’s understanding of microbiology and laboratory learned experimental techniques.
Introduction In class, a series of experiments were performed that pertained to the enzyme known as catalase, which converts hydrogen peroxide into oxygen. Due to peroxide being toxic to the tissues of both plants and animals, both possess the enzyme catalase, which breaks into two non-toxic compounds: water and oxygen gas. Enzymes are proteins that react to certain substrates to create a product, and continue doing so afterwards. Methods and Materials To test reactions between catalase and hydrogen peroxide, groups of three to four people were formed.
The overall project goals and central questions that has to do with the project is mostly trying to determine the isotonic concentration of the salt in potato roots and the use of the ideal soil salt conditions for the potato plant growth. In part 2, we had to test the enzyme activity that is in the was involved in the potato, so we can also determine the ideal soil pH conditions for the potato plant growth. In part 3, we were able to test absorb the leaf pigment at various wavelengths that determine the optimum light absorption conditions and was able to make recommendations for the light conditions that would be used un greenhouses. The goal of the first project is to determine the ideal soil salt for potato roots and we can relate this to the project is to find out if Solution A or Solution B has more solute in it. The goal of this project is to determine the meaning of Osmosis.
This experiment is an attempt to investigate the amount of water potential across root storage plant species. The root storage plant species that shall be used are the carrot and the potato and the method that shall be used is known as Chardakov’s method. Water potential is the tendency of water to enter or leave a cell. Water moves from an area or region of low water potential to an area of high water potential. It is important to note that the highest water potential is 0(the water potential of pure water) and the other water potential values are in negative numbers .
RESEARCH QUESTION Which one has a higher rate or respiration between dicotyledonous (peas) and monocotyledonous (maize) seeds and what is the effect of temperatures (room temperature, 40, 60) on the rate of respiration as determined by oxygen usage estimated with a respirometer? AIM The aim of this experiment is to investigate which seed has a higher rate of respiration and how different temperatures (room temperature, 400C, 600C) affects the rate of respiration of dried, fresh and germinating monocotyledonous (peas) and dicotyledonous (maize) seeds.