Coomassie Essays

ACIDITY TEST INTRODUCTION: Acidity is the total amount of hydrogen ion present in the food sample with the expectation of those bound to alkaline ions. The hydrogen ion can be either attached to acids or in the form of free ions or anions. Titratable acidity is different than total acidity although at times both terms are used to mean the same thing total acidity is the total amount of organic acids in the food sample. This all acids (tartaric, oxalic acid, citric acid, sulfuric acid, lactic acid

with Coomassie blue. Finally, image the gel and membrane to determine the response of the protein. We hypothesize that when placing the cells at 42°C there will be more gene expression in DnaK. E. coli was placed in media and was harvested during log phase. Separated the samples then exposed it to heat shock. The temperature was 42°C and run it for 1 minute, 3 minutes, 5

Quantifying this protein content can help scientist later research biochemical activity of the cells. In this method the Coomassie Blue binds to proteins to form a blue complex whose absorbance is directly proportional to protein concentration. The protein content was measured in micrograms per milliliter. Using the protein content of each cellular fraction a standard curve

experiment are; SDS-PAGE protein electrophoresis, coomassie staining and western blotting. SDS-PAGE is a regularly used procedure to separate proteins via electrophoresis using sodium dodecyl sulphate (SDS) to break down proteins, and a medium of discontinuous polyacrylamide gel. SDS-PAGE can be used for many different uses such as; finding important proteins in a sample and figuring out how much each protein is in a sample (Experimental Biosciences). Coomassie staining is used on SDS-PAGE gels after proteins

I loaded the 15ul of the five serum proteins: Cow serum, Serum albumin, Transferrin and Gamma globulins. The electrophoresed gel was placed on the voltage machine at 110 volts for 1.5hours. We removed the gel from the sample well and place the Coomassie Blue stain. The gel was detained with vinegar by the professor. The next class period, we observed the sample well

Abstract: Many species must evolve to survive and adapt to their environment that is constantly changing. By looking closely at the protein structure of a species and understanding the DNA sequence, researchers can learn similarities and differences between species. This can give us a better understanding of how evolution works. This experiment will examine four samples and their actin and myosin amounts. Actin and myosin are used for muscle contracts and will therefor allow us to see how each sample

amount was in E3 fraction with 29.61 ug and its specific activity was calculated to be 463791.4 RFUs/mg. Figure 4: SDS-PAGE Gel of rGFP samples (G0, G3, GCE, W2, W3, E2, E3, ladder) using 12% Resolving Gel and 5% Stacking Gel and stained with Coomassie Blue Figure 4 showed the SDS-PAGE gel made with 12% resolving gel and 5% stacking gel. SDS-PAGE gel was used to analyze the purity of rGFP from a crude extract, and the relative molecular weight. Standard molecular weights of ladder were 97.4, 66

The goal of this experiment is to see the anti-cow antibody bind to cow serum only, and we expect to see the anti-cow antibody bind to the spot that had the cow serum. The system we used is the serum from Cow, Horse, Goat, Sheep, and Donkey, Chicken. In order to able to detect and analyze proteins based on their ability to bind to a specific antibody, the SDS-PAGE and Western Blot was performed. SDS Polyacrylamide Gel Electrophoresis (SDS-PAGE) is a very common technique used to separate proteins

Coomassie G-250 is doubly protonated in acidic conditions and appears red in color; however, when bound to the basic amino acids of the protein, the dye shifts to the anionic blue form. As the protein and dye interact, an electron is donated to the charged

electrophoresis is a process which separates proteins based on molecular weight. The purpose of this method is to separate out the lysozyme by its weight. The weight is known to be 14.6Da. To view the protein separation, the gel was placed into coomassie blue to develop a series of bands to detect the protein by its

Food labels are trusted to indicate their nutritional value and content. The Canadian Food Inspection Agency set standards with food production companies to tolerate no more than a 20% error margin. These standards are set to ensure that the nutritional value and content indicated on the label are as close as possible to what they actually contain. In this lab, a Beer-Lambert Law graph of absorbance versus protein concentration is obtained using BSA (bovine serum albumin) standard curve dilutions

The purpose of this experiment is to learn about the principles of protein assays as well as to learn how to utilize the Beer-Lambert Law by doing various calculations such as how to calculate absorbencies, concentrations, and extinction coefficients. According to the Beer Lambert Law, absorbance is proportional to path length and concentration. For this experiment we will be learning how to use a spectrophotometer which measures transmitted light intensity. Spectrophotometers measure wavelength

INTRODUCTION Peptic ulcers are also known as “ulcus pepticum”. An ulcer is defined as a non malignant mucosal lesion of the stomach. It occurs due to exposure of stomach and duodenum to pepsin and gastric acid. Peptic ulcer is due to imbalance occurs between aggressive factors like acid, pepsin, H. pylori and defensive factors such as gastric mucus, nitric oxide and growth factors bicarbonate ions and prostaglandins, mucosal blood1. Local mechanisms implicated in mucosal defence are mucus-bicarbonate

1-6 was then placed under UV-light or onto a transilluminator for the visualization of the fluorescent modification. Then the visualized gel was documented by taking a picture in Gene map program. Afterwards, the visualized gel was stained with Coomassie Brilliant Blue protein stain. Staining was done for 30 to 60 mins, within this time the staining solution was recycled after 15 mins. Then destaining was done in a destaining solution and the solution was recycled several times until the background

Plasma derived Factor VIII (Lyophilized powder from human plasma produced by Biotest, IBRF) was used as a positive control. The SDS-PAGE gels were stained by Coomassie Brilliant Blue R-250 (Merck, Germany). The SDS-PAGE gels were transferred to PVDF membrane (Roche, Germany). The membranes were incubated overnight at 4°C with 5% skim milk (Merck, Germany) in PBS as blocking solution, and washed in PBS. The membranes

titel achterkant Voorwoord Samenvatting Table of Contents List of abbreviations 1 1. Introduction 2 1.1 Biobased products 2 1.2 RuBisCO 3 1.3 Isochrysis galbana 4 1.4 Tetraselmis sp 4 2. Methods 5 2.1 Size Exclusion Chromatography 5 2.2 SDS-PAGE 6 2.3 Bradford protein assay 7 2.4 Ion Exchange Chromatography 7 2.5 Soxhlet extraction method 8 2.6 Kjeldahl method 8 3. Materials 9 3.1 Size Exclusion

Their visualisation was performed using Coomassie brilliant blue (G-250). GS-800TM Calibrated Densitometer (BioRad, California, USA) was used to scan the gels and the resulting image file was assayed using the Quantity One analysing software (BioRad, California, USA). Molecular masses were estimated

Sodium dodecyl sulfate polyacrylamide gel electrophoresis also known as SDS-PAGE is one of the methods for determining the molecular weight of unknown proteins. SDS is an anionic molecule which denaturizes proteins and brings it back to its’ primary structure and it also provides a negative charge to the uncharged molecule. The SDS-PAGE enables the separation of proteins based on their sizes. The larger the size of the protein, the harder it is to travel through the gel thus heavier proteins stay

Materials and Methods Cloning E.coli Library Bacteria and Mysterious Bacteria The gradients of the media used for cloning E. coli bacteria included nutrient agar, ampicillin and arabinose sugar; X-gal was an extra gradient in unknown bacteria media. X-gal is an organic compound that consists of galactose linked to a substituted indole, it is used to detect β – galactosidase activity by yielding a blue compound. A purified E. coli library bacteria, containing a DNA fragment of the pG10 plasmid, was

Analysis of minced meat samples using Polymerase Chain Reaction (PCR) and Gel Electrophoresis to investigate adulteration in the product Food Traceability and Genomics John Barry   Title: Analysis of minced meat samples using Polymerase Chain Reaction (PCR) and Gel Electrophoresis to investigate adulteration in the product Name: John Barry Date: 7/10/14 Aim: The aim of the experiment was to examine minced meat samples for adulteration by amplifying extracted DNA using a PCR method. Gel