Ascorbic Acid
ISOLATION OF CAROTENOIDS FROM TOMATO, PAPAYA AND WATERMELON
EVALUATION OF THEIR ANTIOXIDANT PROPERTY
BY DPPH ASSAY
RVS College of Pharmaceutical Sciences
Sulur, Coimbatore- 641402
Tamilnadu
ABSTRACT: The methanolic crude extract of tomato, papaya and watermelon for their free radical scavengimg property by using ascorbic acid as a standard antioxidant. Free radical scavenging activity has been evaluated using DPPH (1,1-diphenyl-2-picryl- hydrazyl) free radical. Among these three the antioxidant activity of Papaya () was the strongest, followed by watermelon (Cucurbita maxima) and tomato (Solanum lycopersicum.) of free radical scavenging activity with the DPPH method. All the methanolic extracts exhibited antioxidant activity significantly.
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The upper lycopene-containing organic layer was removed by means of a pipette and collected in test tube. Extraction was repeated. The extracts were combined, washed with 15mL saturated aqueous sodium chloride (NaCl) and removed the aqueous wash with a micropipette. The extract was washed with 10mL of 10% aqueous potassium carbonate (K2CO3) and removed the aqueous wash. The lycopene-containing organic layer was dried with a drying agent (calcium chloride). The excess solvent was allowed to evaporate at room temperature for a few minutes in the dark. The tubes containing lycopene extracts were covered with aluminum foil and stored in freezer until further analysis …show more content…
Silica plates (MERCK) were prepared by drawing a pencil line 1 cm from the bottom of the TLC plate. Samples were spotted using glass spotters. The organic solvent (9:1 petroleum ether and dichloromethane) was used [35]. TLC plate was placed in the tank for 5-10 min. The edge of plate was marked to indicate how far the solvent traveled up the plate. TLC plate was dried in hood, the pigments were marked with a pencil and the plate was analyzed under UV lamp.
Rf value = distance from origin to component spot (cm)/ distance from origin to solvent front (cm)
FOURIER TRANSFORM INFRARED (FTIR) SPECTROSCOPY
Fourier Transform Infrared (FTIR) Spectroscopy was performed on purified lycopene samples. FTIR Spectrometer (BRUKER, Germany) was used. The liquid samples are sandwiched between two plates. The plates are transparent to the infrared light and do not introduce any lines onto the spectra. Standard spectra of the samples were collected individually [36].
RESULTS
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