Chromatography is a separation technique, that was developed by an Italian-born botanist called Mikhail Tswett, who was from Russia in 1906. Chromatography was used to study plant pigments as well as, separate parts of colourless mixtures, to identify and name amino acids present within. The word chromatography originates from the Greek word ‘khroma’ which means colour. There are several forms of chromatography but they all work on the same principle. Some types of chromatography are; paper, thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and gas chromatography (GC). However, paper chromatography is used the most often. Chromatography is used in many different places in life, including; pharmaceutical companies …show more content…
When the chromatography paper is placed in the solvent, the solvent penetrates the paper by capillary action. Capillary action is the movement of liquid within the spaces of the material, due to adhesion, cohesion and surface tension. The adhesive intermolecular forces between the liquid and solid are stronger than the cohesive intermolecular forces in the liquid. Solubility also affects the chromatographic results as, the more soluble the pigment is the further it will travel up the chromatograph paper. Another affecting factor is the molecular weight, the substances with a smaller molecular weight will travel higher up the paper. Polarity also affects how the substances move. Substances with a higher polarity will travel fast up the paper but will not go as far. Polarity has an effect on how attracted the chemical is to another substance. As some molecules have a positively charged side and negatively charged side, the opposites attract. When the charge difference is bigger, it means that the molecule is more polar. When the polarity of the solvent is increased, the components of the mixture move faster during the chromatography experiment. Therefore, if the polarity is decreased the components will move
Organic modifiers are used to change the retention time of different analytes. Organic modifiers lower mobile phase polarity. By increasing the amount of water lead to the repulsion of hydrophobic analytes out of the mobile phase. The hydrophobic analytes are pushed onto the stationary phase where they reside for duration up to the partitioning into the mobile phase. When ionic analytes exist in the sample, the addition of ion and buffer to the mobile phase are necessary.
From the data obtained in Tables 1-3, we were able to plot multiple graphs using excel. These graphs give a better representation of the data as seen in Figures 1-9. It can be seen that each figure shows a slight increase in CO2 production, which signifies a possible change in metabolic rate. Figures 4 and 7 show a relatively large change between the control and fox urine. The changes in slope between theses two are 0.0267 for Figure 4’s slopes and 0.0192 for Figure 7’s slopes.
Title: Determining Polarity, Gabriella Anzelc, Mrs. Stockman Objective(s): Comparing the solubility of different compounds and determining their polarity of them based on the solubility. Hypothesis: Create a hypothesis that predicts the polarity of iodine solution, vinegar, vegetable oil, salt, water, and rubbing alcohol. If the compounds (iodine solution, vinegar, vegetable oil, salt, water, and rubbing alcohol) dissolve in water, the compounds are
Materials: 100 mL plastic beaker blue crayola marker magenta crayola marker whatman filter paper 10mL tap water plastic pitcher of water blue scissors brown school paper towels SAFE-T plastic view-thru ruler 0.5 mechanical pencil clock Using Paper Chromatography to Separate Ink-Lab sheet Method: Using a small plastic pitcher filled with room temperature water, pour 10mL of the water into a small plastic beaker. If the walls of the beaker are wet be sure to dry them with a paper towel. With your scissors cut the Whatman filter paper into two strips, then cut one end of each strip into a point. Measure approximately two centimeters above the tip of the point on one of the pieces of filter paper with a ruler and mark a straight horizontal
Leah Romero 10/30/2017 Conclusion Lab 3 Chem 102L In lab 3, fundamentals of chromatography, the purpose was to examine how components of mixtures can be separated by taking advantage of different in physical properties. A huge process in this lab was paper chromatography, which was used to isolate food dyes that are found in different drink mixes. The different chromatograms of FD&C dyes were compared to identify which dyes are present in each of the mixes.
TLC was used to identify the actual unknown product as well as other products/reactants present in the filtered solution. The procedure was conducted by placing a TLC plate in a developing chamber that is filled with a small amount of solvent. The solvent cannot be too polar because it will cause spotted compounds on the TLC plate to rise up too fast, while a very non-polar solvent will not allow the spots to move. The polarity of the spots also determines how far it moves on the plate; non-polar spots are higher than polar ones. After spots on the TLC form, the Rf values are calculated and used to analyze the similarity of the compounds.
Jaspreet Singh Professor Paratore Biology 1 November 1, 2014 Spectrophotometry Identifying Solutes and Determining Their Concentration Statement of the Exercise or of the Problem The purpose of the lab experiment was to attain the following objectives: • Learning to Operate the Spectrophotometer • Construct absorption spectra for cobalt chloride and chlorophyll. Hypothesis If greater and higher concentrations of cobalt chloride are added to each solution then greater amounts of light would be absorbed by each solution. Thus a liner relationship will result in which the absorbance of a substance would be proportional to its concentration, which will be depicted, in a linear graph.
INTRODUCTION A gas chromatograph (GC) can be utilized to analyze the contents of a sample quantitatively or in certain circumstances also qualitatively. In the case of preparative chromatography, a pure compound can be extracted from a mixture. The principle of gas chromatography can be explained as following: A micro syringe is used to inject a known volume of vaporous or liquid analyte into the head or entrance of a column whereby a stream of an inert gas acts a carrier (mobile phase). The column acts as a separator of individual or chemically similar components.
Scientific American says that “In a liquid, the molecules (small individual particles that can have positive and negative charges on their surfaces) can, just like magnets, attract and repel one another. In the case of a water molecule surrounded on all sides by other water molecules there is roughly the same amount of pulling and pushing force”. With the whole milk at 1 minute with just food coloring the circumference was 1 centimeter, at 2 minute it was at 2.5 centimeters wide and at 3 minutes the width was 3.5 centimeters. The half and half milk at 1 minute the width was at .7, at 2 minute the width was 1cm. 3 minutes the width was at 1.4.
Introduction Chevron Phillips Chemical Company is the major producer of Cyclohexane. This successful company hoses the three largest cyclohexane plants in the world. Many are puzzled by how the production of cyclohexane seems to have become stagnant. Perhaps this is due to the cost of benzene increasing or the demand increasing. Through thorough investigation, the answer to this question and many more can be answered.
Properties of Substances Express Lab 1)The purpose of this lab was to compare the physical properties of different types of solids and how the properties of solids are determined by their intermolecular forces and their intramolecular bonds. Then we were to classify each type of solid as either ionic, metallic, non-polar molecular, polar molecular, or network. Paraffin wax classified as a non-polar molecular, Silicon dioxide was classifies as a network, Sodium chloride was classified as ionic, Sucrose was classified as polar molecular and Tin was classified as metallic. (2)The intermolecular forces that are present in Paraffin wax are dispersion forces, because it is non-polar and carries a negative charge. Followed by Sucrose that has
The ammonia: 1-butanol (1:1) solvent was the appropriate solvent to use for the column chromatography of food dye because it exhibited the properties of a good solvent system. A total 8 colored eluents were collected. The eluents had colors of pink, dark red, dark blue, dark green, light green, yellow, orange and light yellow respectively and
The stationary phase is the phase that does not move and the mobile phase is the one that does move. The mobile phase moves through the stationary phase picking up the compounds to be tested. As the mobile phase continues to
DETERMINATION OF PERCENTAGE ETHANOL IN BEVERAGES 1. Introduction to Gas Chromatography Gas chromatography is a very powerful separation technique for compounds that are reasonably volatile. The components of a sample partitions into two phases, the 1st of these phases is a immobile bed with a great surface area, and the other is a gas phase that permeates through the immobile bed. The sample is evaporated and passed by the mobile gas phase or the carrier gas through the column. Samples separates into the stationary liquid phase, based on their solubilities at the given temperature.
Introduction Drug use in sports has always been a controversial issue. With athletes pushing for the top podium position, performance enhancing drugs can be extremely enticing. One of the main types of drugs used by athletes are stimulants such as cocaine, amphetamines or ecstasy. These can create unfair advantages in sports. To keep sports even and fair, certain drugs became prohibited.