Dilution Factor Experiment

733 Words3 Pages
A simple dilution is the combination of a liquid material of interest with a solvent liquid to achieve the desired concentration. The dilution factor refers to the ratio of the volume of the initial concentrated solution to the volume of the final dilute solution. The diluted material must be thoroughly mixes well to achieve the true dilution. The formula, C1V1 = C2V2 is widely used in dilution to calculate the concentration or volume of the solution either is on before or after the dilution. V1 = Volume of stock solution needed to make the new solution C1 = Concentration of stock solution V2 = Final volume of new solution C2 = Final concentration of new solution The dilution factor can be used alone or as the denominator of the fraction.…show more content…
The different level of blue colour of the solutions after adding the iodine solution show the different concentration of starch concentration in it. The change in colour is due to the formation of polyiodide chains from the reaction of starch and iodine. The amylose in starch forms helices where iodine molecules assemble, forming a dark blue or black color. 1ml of starch solution with concentration 5000 are added to test tube A. 1 unit volume of the starch solution to be diluted plus 9 unit volumes of the solvent medium. Hence, 1+ 9=10. 1 : 10 is the dilution factor. The test tube is shaked will before 1 ml solution is transferred to test tube B. The concentration of starch of the 1ml solution from test tube A is 500 after dilute with 9ml distilled water in test tube A. The 500 of starch solution become more dilute after added into 9ml test tube B, which become 50 . The concentration of solution in the few next test tube decreases in such way. The dilution factor, 5000 ratio to 50 is 1 : 100 in test tube B while the dilution factor in test tube C, 5000 ratio to 5 is 1 : 1000 and so…show more content…
The colour showed in test tube A is dark blue but the rest are all nearly yellow. This mean there must be an error when the experiment is carried out during the dilution and transfer of solution from one test tube to another. With each sequential serial dilution step, transfer inaccuracies can lead to less accurate and less precise dispensing. To relieve this error possibility, longer mixing times are required, which increases the time required to perform the serial dilution to allow the solution to be thoroughly mixed well. Theoretically, longer mixing times are required for more dilute samples,. However, this is usually not compensated for and unequal concentrations which are usually used to innoculate the next

More about Dilution Factor Experiment

Open Document