Hydrochlorthiazide Lab Report

ABSTRACT

Floating bioadhesive tablets of hydrochlorthiazide were developed to prolong gastric residence time leading to an increase in drug bioavailability. Tablets are prepared by direct compression technique using polymers Xanthum gum, Chitosan, Eudragit L- 100, Magnesium stearate USP-NF (Avicel PH 102), Microcrystalline cellulose Ph 102, Talc, Microcrystalline cellulose Ph 102 and sodium bicarbonate. Tablets were evaluated for their physical characteristics viz., hardness, thickness, friability and weight variation, drug content and floating properties. Gas generating agent plays an important role in floating lag time and drug release. The best formulation subjected for kinetic treatment. i.e., zero order, first order, peppas,

STANDARD CURVE IN 0.1 N HCL
Accurately weighed 10 mg of drug (hydrochlorthiazide) was first dissolved in10 mL of methonal in 100 mL of volumetric flask to make a concentration of 1000 μg/mL (primary stock solution). 1 mL of primary stock solution was pipetted out into 10 mL of volumetric flask and volume was adjusted with water to make a concentration of 100μg/mL (secondary stock solution).From the secondary stock solution various concentrations such as 1, 2, 3, 4, 5…..10 μg/mL were prepared for calibration curve. Standard curve was plotted by taking absorbance of secondary stock solutions in UV double beam spectrophotometer at 240 nm.
Compatibility Studies
Compatibility with excipients was conformed by carried out I R studies. The pure drug and its formulations along with excipients were subjected to IR studies. In the present study, the potassium bromide disc (pellet) method was employed.
The objective of drug/excipient compatibility considerations and practical studies is to delineate, as quickly as possible, real and possible interactions between potential formulation excipients and the API. This is an important risk reduction exercise early in formulation development. Homogenous mixtures of drug and excipients were prepared and filled in glass vials and self-seal LDPE (Low Density Poly Ethylene)