50mL HCl was added to the calorimeter, which was covered with the lid and inserted with a probe. Readings were collected and then 50mL NH4OH was swiftly added to the solution, with the stir bar mixing the reaction constantly. The initial and max temperature temperatures were recorded and the change in temperature was recorded. The data was saved onto the USB. Regarding reaction 4, the duration was changed to 480 seconds.
After adding three boiling chips, 10 mL of 48% hydrobromic acid was also added to the round bottom flask and swirled for 15 seconds to reactants in the flask. The reactants were clamped to a ring stand and a pre-set reflux apparatus with clear hoses attached to the condenser. The voltage regulator was set to 40 to begin water flow through the condenser and the application of heat, so the solvent can boil. The reaction was set to reflux for 30 minutes. Upon completion, the round bottom flask cooled for three minutes in a beaker filled with room temperature water and again in a beaker with ice cold
At certain time intervals, 1 ml of sample was withdrawn and immediately same amount of fresh medium (37±0.50C) was replaced. The UV absorbances of samples were measured at 294.5 nm by UV spectrophotometer and drug release was calculated. 6.4.9. Scanning electron microscopy
An equivalent volume of saline solution was added (NaCl 0.16 M; pH = 7) and it was mixed completely. The resultant solution was centrifugated for 30 minutes at 9000 rpm at 4ºC in a rotor Beckman JA-20, eliminating the pellet. The supernatant was collected to obtain the lipoproteins by gradient density ultracentrifugation: 10ml of the yolk solution were taken and then 0.9 g of potassium bromide (KBr) was added. The bromide was dissolved with a smooth agitation, with care not to denature the proteins. Straight afterwards, saline solution was added (NaCl 0.16 M, pH=7) to the 10ml of the yolk solution with a Pasteur pipette, avoiding the sample diffusion, forming two phases and filling the tube completely.
Microspheres found to be sustained over 8 hrs.11 Propranolol hydrochloride bilayer matrix tablet was developed and it contain as the fast release layer (sodium starch glycolate) and sustaining layer (EC, Eudragit RLPO and Eudragit RSPO) by direct compression technique. The formulation gave an initial burst effect to provide the loading dose of the drug fallowed by the sustained release for 12 h from the sustained layer of matrix embedded tablets. In vitro dissolution kinetics followed the Higuchi model via a non-Fickian diffusion controlled release mechanism after the initial burst release. Statistical analysis (ANOVA) showed no significant difference in the cumulative amount of drug release after 15 min, but significant difference (p < 0.05) in the amount of drug released after 12 h from the optimized formulation was
50 mL of distilled water is added to the 250 mL beaker and 10 mL of HCl is pipetted into the beaker. The beaker is placed on the magnetic stirrer and a stirring bar is placed inside the solution. The beaker and the magnetic stirrer are placed under the burette. The support ring is attached to the laboratory stand and a pH Sensor is positioned on it so that it is immersed in the HCl solution. Make sure the stirrer will not hit the pH probe.
In this study, we attempted to enhance the systemic bioavailability of VDF via its formulation within vaginal suppositories. Witepsol H15 and Suppocire NA50 were adopted as lipophilic suppository bases while polyethylene glycol 4000/400 and glycerogelatin were used as hydrophilic suppository bases. The effect of different base types and/or the incorporation of bioadhesive polymer on in vitro release of VDF were evaluated. The in vivo fate and organ biodistribution of VDF following intravaginal (IVG) administration were also investigated. VDF release from water-soluble bases was higher than that from lipophilic bases.
ABSTRACT: The mathematical model for absorption of cosmetics drug formulations through human skin is studied. The study is concerned with the structure of the skin, since it has structures that enhance absorption of drugs through its permeable. Liposome cosmetic drug is used in this study. A partial Differential equations describing Fick’s second law is solved using Finite Difference Method. The equation is used to determine mathematically absorption these cosmetic drug formulations through the skin.
GENETIC CONSEQUENCE OF CALCIUM PHOSPHATE MICROSTRUCTURE OF BIOCERAMICS Abstract: sympathetic of relations between cells and biomaterials is a gigantic parameter for humanizing tissue engineering and regenerative medical fields. Many dissimilar materials have previously been tested and it has been traditional that surface distinguishing is a parameter that influences cell responses. The intend of this work was to characterize calcium phosphate discs containing an assortment of HA/β-TCP and explicit microstructure. First outcome show that chemical symphony and solidity parameters amend surface equipment. Secondly, cells were cultured and morphology, practicability, and discrimination were studied.
But floating drug delivery remains in the stomach for longer period of time to their increased gastric retention. In many cases of modified release capsules it has been observed that the major portion of drug releases in the colon rather than stomach. However prolongation in the GRT may sustain the release of drug.30 Site specific drug delivery: Some drugs like furosemide and riboflavin are having their absorption site in the upper small intestine so they are typically formulated as floating dosage forms. Floating drug delivery serves an excellent drug delivery for the treatment of Helicobacter pylori, which causes gastric and peptic ulcers. The treatment needs high drug concentration of drug to be maintained at the site of infection that is within the gastric mucosa.