The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate. All enzymes are under the class of protein biomolecule. Amino acids are the basic units that are combined to make up an enzyme.
Enzymes are proteins that significantly speed up the rate of chemical reactions that take place within cells. Some enzymes help to break large molecules into smaller pieces that are more easily absorbed by the body. Other enzymes help bind two molecules together to produce a new molecule. Enzymes are selective catalysts, meaning that each enzyme only speeds up a specific reaction. The molecules that an enzyme works with are called substrates.
The rates of absorption were calculated using a spectrophotometer in 20 second intervals up to 120 seconds. It was hypothesized that the optimal pH for the enzyme was pH 7 while the 1.0 ml peroxidase would have the best reaction rate. At the end of the experiment the results prove the hypothesis to be incorrect. INTRODUCTION Enzymes are proteins that allow a reaction to speed up. These proteins are made up of monomers known as amino acids.
The amino acid residues known for their acidic form will function as the proton donor and the residues that form a base will act as a proton acceptor. The low bond dissociation energy formed by hydrogen bonding is what makes transferring of a proton more favorable within an enzyme. This allows the enzyme to achieve its characteristic features as a catalyst without undergoing any chemical reactions
Lab Report -- Relationship on Enzyme activity and substrate concentration Research Question: Is the more concentrated the substrate of hydrogen peroxide is, the shorter the time taken for the paper disc to rise from the bottom of the beaker? Aim: The opposite of hull hypothesis Background Information: This experiment aimed to investigate on the relationship of the substrate concentration and enzyme activity. Enzymes are proteins produced by a cell that acts as catalysts to increase the rate of a specific chemical reaction without changing the reaction itself. Under some conditions, substrate will bind to the active site of an enzyme and form an enzyme-substrate complex. The enzyme would fasten the chemical reaction and the substrate will
Metabolism is also regulated by hydrophobic hormones which enter their target cells and are able to interact with intracellular receptor molecules. The movement of substrates into the cells compartments is also controlled by the energy charge. The charge depends on the amounts of ATP, ADP and AMP which are all relatively regulated.
In aqueous solutions of protein, there is a cooperative hydrogen–bonded structure.  When sugar is added to the protein solution, the OH groups of sugars may also compete for hydrogen-bonding.  Now we have to consider the respective interactions between protein, water and additive (sugar) molecules. The additive interacting more strongly with protein than with water will tend to stabilize the denatured states by the formation of protein additive complexes. They will, therefore, have a denaturing effect.
Introduction: Enzymes are biological catalysts that increase the rate of a reaction without being chemically changed. Enzymes are globular proteins that contain an active site. A specific substrate binds to the active site of the enzyme chemically and structurally (4). Enzymes also increase the rate of a reaction by decreasing the activation energy for that reaction which is the minimum energy required for the reaction to take place (3). Multiple factors affect the activity of an enzyme (1).
4.3) Briefly explain what you understand by specific activity of an enzyme and how you could measure it. (P 4.3) Specific activity is a term utilize to measure the rate of reaction of an enzyme with a substrate. Specific enzyme activity is a measure of enzymes purity and quoted as units/ mg. the value becomes huge as an enzyme preparation becomes purer since the amount of protein (mg) is typically less, but the rate of reaction stays the same/ may increase due to reduced interference/ removal of inhibitors. SPECIFIC ACTIVITY MEASURING ENZYME KINETICS Enzymes are proteins that act as catalysts in reducing the amount of activation energy for a reaction to take place, activation energy that is needed for get reactions begins since several reactions. Do not take place at all.
Research Question How does the change in temperature affect the effectiveness of protease on breaking down egg whites? Introduction Enzymes are a substance known as biological catalysts, this basically means they can, without being used up, speed up chemical reactions. Enzymes are made up proteins that create elaborate shapes that smaller molecules, such as protein particles, can then fit in. These areas that allow the molecules to fit in are called active sites. Some different enzymes such as protease, amylase and lipase, all work well in different conditions, and in different parts of the digestive system.