Acne Medication Investigation Essay

GRACE COLLEGE

Acne Medication

One Research Task

Calvin Watkins

2014

Table of Contents

Introduction 2

Aim 2

Hypothesis 2

Variables 2

Apparatus 3

Method 3

Results 5

Analysis of Results 7

Review of Literature 8

Conclusion 8

Acknowledgements 9

Bibliography 9

Introduction

For my One Research Task, I will investigate the effectiveness of Acne medication on the growth of acne related bacteria. In this investigation I will be using three types of active agents used in acne medication, these are the antibiotics Tetracycline and Clindamycin and the bactericidal compound Benzoyl peroxide. I understand that Tetracycline and Clindamycin are prescription antibiotics but due to the minute amount of the drugs I will be using, I am able to acquire

Seal the new petri dishes and place in an incubator at ±37˚C for 5 days

9. Store the old petri dishes in a sealed plastic bag for the next 5 days

Preparation of medicine solutions and control

1. Label three test tubes A, B and C

2. Place one capsule of Doxycycline and Clindahexal in test tube A and B respectively

3. Crush one tablet of Purbac DS and place in test tube C

4. Add ethanol and distilled water to each test tube according to the data given in table 1.1 below

5. Stir/whirl the solutions until all the powder from the tablet has dissolved into the solution

6. The solutions must be stored in a refrigerator if they are being stored for a long period of time and may need to be re-stirred before the solution can be added to the filter disks

Preparation of filter disks

1. Punch disks of filter paper using a paper punch

2. Add the various amounts of medication solutions as shown in table 1.2 below to each filter disk using a micropipette

3. Place filter disks in a sealed container and leave for 24 hours

Suspension of bacteria

1. Unseal the petri dishes

2. Add 1000 microliter of 0.8% of saline solution to the centre of the first petri dish using a micropipette

3. Sterilize a Drigalski spatula by flaming with