In the Oxidative fermentation tube the media was a differential media that helps determine whether specific bacteria can oxidize or ferment to metabolize glucose. Citrate test checks to see which bacteria could citrate as the only source of carbon. A positive test shows that an alkaline environment ia created and that the pH level rose. The color of the media changed from green to blue if its positive. The Bile Esculin agar test has its medium as selective and differential.
After a gram stain was done unknown #257 was identified as a gram positive organism because when observed under the microscope the organism appeared purple with cocci in clusters. The organism was also catalase positive which means that it produced enzyme catalase and bubbled when hydrogen peroxide was added to it. Three test were conducted based on the result of the gram staining procedure. Blood agar with a Novobiocin disk was chosen as well as DNase (DNA) and Mannitol Salts (MSA) agar. The Blood agar is a bright red, opaque plate and the streaking or the inoculation technique was a modified streaking for isolation with a heavy quadrant one.
Compare the result to the chart on the back of the urinary pH test strips bottle, and record data. Clean the stirring rod with water before moving on to the next test tube. Repeat this process for each increment (2 mL, 3mL, 4mL) Figure #1: Picture of bean solution mixed Figure #2: Picture of materials needed for the with alpha galactosidase experiment Safety considerations: Be careful with the beakers, glass stirring rod, and test tubes, as they could break easily and can cause cuts in the skin. DCP: A scatter plot will be used to display how the amount of alpha galactosidase (measured in mL) in the bean solution affects the glucose concentration (measured in mg/dL) and error bars to show the standard deviation. A line of best fit will be used to show the relationship between the glucose concentration and the amount of alpha galactosidase.
Screening to separate the large contaminants from the water b. Coagulation to attract small contaminants c. Sedimentation where water sits and finishes coagulation d. Filtration to remove any small remaining contaminants and particles e. Disinfection by disinfecting chemicals such as chlorine to kill microorganism or remaining bacteria 2. Explain the differences between primary, secondary, and tertiary sewage treatment. a.
Proton pump inhibitors (PPIs) are a group of medicines, such as lansoprazole and omeprazole, that work on the cells that line the stomach, reducing the production of acid. The stomach normally produces acid to help with the digestion of food and to kill germs (bacteria). This acid is corrosive so the body produces a natural mucous barrier which protects the lining of the stomach from being eroded. In some people this barrier may break down and allow the acid to damage the stomach, causing an ulcer. In others, a problem may occur with the muscular band at the top of the stomach (the sphincter) that keeps the stomach tightly closed.
Hence, a calcium chloride and cotton were filled inside a drying tube. The condenser was wrapped with parafilm and a paper towel to avoid moistures from entering. The reagent will act as nucleophilic addition to acetone and work up with hydrochloride acid to synthesize 2-methylhexanol. Throughout this process, the solution turns dark grey and develop white precipitates. This step indicate that Grignard reagent was generated, and the extra white precipitates were magnesium.
Since the solvent is nonpolar, we would expect carotene to have the lowest Rf, then xanthophylls, and chlorophylls would have the highest. As discussed before, this is because chlorophylls are the most polar of the three and carotenes are the least polar of the three. Based on my results, I would say that the packing and running of the column was effective in separating the pigments as there were quite different Rf values for the original than for the other two colors and the band for the yellow was nothing like the bands for the green. There were no limitations in procedure which negatively affected the end results, so there is nothing I would change to it going
This transformation usually occurs within plasmids, which are closed circular molecules made up of double stranded DNA. The function of the plasmid is to provide bacteria with genetic advantages such as antibiotic resistance. In this lab, the plasmids provided the ampicillin resistance and the fluorescence. If the bacterial cells are grown in the presence of the antibiotic ampicillin then only the cells that took up the plasmid have the resistance gene. As a result the resistance gene will have to keep the plasmid and the GFP gene.
The vacuum we made was to remove the air from the discs and fill it with the sodium bicarbonate solution. Because they now lack the gases that were keeping them afloat, the discs sink to the bottom of the solution. But when light is added shortly afterward, the discs start to float again. Chlorophyll inside the discs absorb the light and make ATP. Despite this reaction, cellular respiration is still occurring within the spinach leaves.
The alcohol also decolorizes the exposed peptidoglycan layer by dissolving away the previously applied crystal violet. A counterstain (safranin or fuchsine)is then added which recolorizes the bacteria red or pink. Then, the example for bacillus could be mixed bacillus-Gram stained. For the spiral can be