Case Study Of Povidone-Iodine

• Povidone - Iodine: Antiseptic solution (B- SEPT), from SAGA PHARMA, Egypt.

• Benzyl Penicillin: In the form of vials containing 1000000 IU Penicillin G, purchased from Misr Company for Pharmaceuticals Industry, Egypt. The vial was dissolved for intramuscular injection using ampoules containing 5 ml sterile water for injection from Egypt Otsuka Pharm Co., Egypt.

• Estradiol Benzoate: In the form of ampoules; each contains estradiol benzoate 5mg. (Folone) purchased from Misr Company for Pharmaceuticals Industry, Egypt. Estradiol benzoate was dissolved in sesame oil to obtain a dose of 5 µg of estradiol benzoate in 0.1 ml sesame oil.

• Avosoya: In the form of hard gelatinous capsules containing avocado/soybean unsaponifiables (ASU) extract

After complete separation of the placenta, cord blood was collected under strict aseptic conditions. Cord blood was directly drawn into a 50 ml tube containing 5 ml of phosphate buffer saline (PBS) pH 7.2 and 2mM EDTA. Cord blood was then diluted 3X with PBS buffer. The mononuclear cell fraction was isolated by loading 30 ml of whole blood onto 10 ml of Ficoll/Paque (Lymphocyte Separation Medium 1.077, Lonza Bioproducts) in 50 ml tubes, then, centrifuged for 30 min at 20oC. Interphase MNCs layer was collected after aspirating and discarding the supernatant. The cells were washed with 20 ml PBS and centrifuged at room temperature. The supernatant was aspirated and the cells were washed with PBS a second time. MNCs were subcultured with culture medium [low glucose Dulbecco 's Modified Eagle 's Medium (DMEM 1.0 g/L glucose Lonza Bioproducts, Belgium) and 10% fetal bovine serum (FBS, Lonza Bioproducts, Belgium)]. Cells were incubated at 37°C in 5% humidified CO2 in CO2 incubator (Heraeus, Germany). Non-adherent cells were eliminated by a half medium change at 1, 2, and 3 days and the whole medium was replaced with fresh medium every week. The cells were grown for 2–3 weeks until 80-90% confluence. The whole adherent cells were then detached by trypsinization with 0.25% trypsin/ ethylene diamine tetra acetic acid (EDTA) (Trypsin 1:250, EDTA 1 mM, LonzaBioproducts, Belgium)