Laboratory Report on Fermentation of Yogurt and Ginger Ale
Introduction
Fermentation is a metabolic process in which an organism converts a carbohydrate, such as starch or a sugar, into an alcohol or an acid. There are two types of fermentation namely ethanol fermentation and lactic acid fermentation. Ethanol fermentation occurs when pyruvate from glucose metabolism is broken down into alcohol and oxygen. In lactic acid fermentation, pyruvate molecules are fermented into lactic acid (Helmenstine, 2014).
Examples of fermented products are yogurt and ginger ale. Yogurt is a sour milk product that is usually served with fruit toppings while ginger ale is a carbonated drink often mixed with alcoholic beverages.
This experiment aims to produce
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Explain the process of fermentation in the Yogurt and Ginger Ale.
Yogurt underwent lactic acid fermentation. During the making of yogurt, the lactose is broken down by Lactobacillus bulgaricus and Streptococcus thermophilus into these two glucose and galactose. The further processing of glucose and galactose results in the end products of lactic acid and acetaldehyde. The production of lactic acid and acetaldehyde decreases the pH which causes the milk to clot and produce the sour taste of the yogurt (Westminster College, n.d.). Ginger ale underwent ethanol fermentation. The yeast breaks down the glucose to form 2 pyruvate molecules. Before pyruvate can be converted to ethanol, it is first converted into an intermediary molecule called acetaldehyde. This releases carbon dioxide. Afterwards, acetaldehyde is converted into ethanol (Study.com, n.d.).
2. What common microorganisms is use in processing Yogurt.
The most common starter culture of yogurt is Lactobacillus bulgaricus and Streptococcus thermophilus. Streptococcus thermophilus is classified as a thermophile growing at 45°C, and higher, and is widely used in the manufacture of yoghurt while Lactobacillus delbrueckii subsp. bulgaricus is homofermentative, produces almost 2% w/v lactic acid in milk, has an optimum temperature of 42° and grows at temperatures of 45°C and higher (Mullan,
Name: Avishak Deb Roy Partners: Leevell Penn, Varugh, Butler Bio 101 Lab Report #1 02.22.2018 Swimming speed of paramecium tetraurelia in different levels of treatment. Introduction Paramecia is a unicellular Protista which are naturally found in aquatic habitats. It is easily cultured in the laboratory. It is oblong shaped and covered with short hairy structure called cilia. Paramecia does not pose any health or ethical concerns and the population can be maintained if there is a food source such as Enterobacter (Biological Foundation 7).
Sucrose fermentation: This fermentation makes energy available for use by microorganisms by anaerobic breakdown of carbohydrates. It can either be an acid or gas. When positive turns red to yellow and can have gas present which form bubbles. H2S production: H2S is a toxic gas produced by the decomposition of sulfur-containing amino acids cysteine and methionine or the reduction of inorganic sulfur compounds by enzymes of certain bacteria. If hydrogen sulfide is formed, it reacts with ferrous salts to form black metal sulfides that can be visualized
A milk-based, litmus broth tube is incubated and observed after 48 hours. Observations include lactose fermentation without gas as well as with gas, the reduction of litmus, casein protein coagulation and casein and protein hydrolysis. These characteristics were all determined based on the color of the solution and the production of a curd, the curds density and the production of a gas. To determine the density of the curd, the tube was slightly turned to see rather or not it was mobile or concentrated towards the bottom. 2.3 Carbohydrate Fermentation of Lactose, Sucrose and
Therefore, acid fat, lactose fermentation, mannitol fermentation were not needed to be performed, because they are selective to a specific thing. As a result, Unknown bacteria “W” was concluded to be gram stain positive, endospore positive, bacilli shape (rod shape), and arranged in chains (strepto-). Test Purpose Reagents Observation Results Gram Stain To determine gram reaction of bacteria.
Carbohydrate fermentation- this test determines whether bacteria have the ability to ferment the carbohydrates; glucose, sucrose, maltose and lactose. This test also identifies if the bacteria produces combinations of
Only Escherichia coli and Enterococcus durans will be the species fermenting. PEA agar isolates Gram-positive species from Gram-negative bacteria.
This is the second step. During fermentation, from a rough dense mass lacking extensibility and with poor gas holding properties, the dough slowly changes into a smooth, extensible dough with good gas holding properties. As the yeast cells grow, the gluten protein pieces clump together to form networks. Hence, the alcohol and carbon dioxide are formed from the breakdown of carbohydrates that are found naturally in the flour. Enzymes present in yeast and flour also help to speed up this reaction.
LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.26.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3.
1% glucose, 1% maltose and 1% lactose all progressively get positive results by changing colours to reddish brown at the end of this experiment. In this case the aldehyde functional group that is present in the products (monosaccharides and some disaccharides) in this reaction is able to reduce copper in the presence of alkali and this produces colour changes while converting to an aldose sugar. Honey is made of fructose and glucose which instantly turned brown after the test-tube was placed in the boiling water because of its active aldehyde and carbonyl group. The copper (II) sulphate present in the Benedict’s solution reacts with electrons from the aldehyde group which results in a redox reaction to from cuprous oxide, a red brown precipitate that seen in all of the above mentioned solutions (Hill, 1982). Beer also gave positive results because it contains aldehydes and ketones (i.e. acetone, trans-2-butenal, furfual) during its beer production process where the sugars are converted through fermentation (Hill, 1982).
Title: How Ph Levels Affected the Fermentation of Beer Hypothesis: The beer will be left with more sugar deposit as the Ph levels increase because alpha/beta -amylase will no longer function. Predictions: Alcohol Percentage Analysis for the Control and the Experimental During this experiment, the pH level was increased, therefore Alpha-Amylase was favored. Due to the nature of Alpha-Amylase cutting randomly through a large carbohydrate molecule, it leaves bigger sugars in the flask, which cannot be digested by yeast. Due to this, less reactions should occur in the experimental, therefore leading to a lower percentage of alcohol production, compared to the control.
For example, fermentation occurs in yeast in order to gain energy by transforming sugar into alcohol. Fermentation is also used by bacteria, they convert carbohydrates into lactic acid. Ethanol fermentation is done by yeast and certain bacteria, when pyruvate is separated into ethanol and carbon dioxide. Ethanol fermentation has a net chemical equation: C6H12O6 (glucose) > 2C2H5OH (ethanol) + 2CO2 (carbon dioxide). This process of ethanol fermentation is used in the making of wine, bread, and beer.
CLAIRE MUNTING 29/01/2018 Criterion C EFFECTS OF SURFACE AREA OF CALCIUM CARBONATE UPON RATE OF REACTION Calcium Carbonate Chips 1 Introduction: Within the current investigation, the effects of the surface area of Calcium Carbonate (CaCO3) in combination with Hydrochloric acid (HCl) upon its rate of reaction. CaCO3, commonly referred to as limestone, is an organic substance and is, in a sense, the crystallised “carbonic salt” of the element, calcium2. In addition to being a salt, the pH level of Calcium Carbonate is 9.91, and it is therefore, a basic substance, due to the fact that it is comprised of a pH level higher than 7, which is neutral3. HCl, however, is the bodily acid found in the stomach of human beings.
Joshua Miller 12/18/17 Fermentation Lab report Introduction The term fermentation refers to the chemical breakdown of a substance by bacteria, yeasts, or other microorganisms, typically involving effervescence and the giving off of heat (wikipedia). Sugars are converted to ethyl alcohol when fermentation happens. In this experiment we determined if yeast cells undergo fermentation when placed in a closed flask with no oxygen. Glucose and yeast are mixed together in a closed flask and allowed to incubate for about one hour.
INTRODUCTION: Lipase also called as triacylglycerol acylhydrolaseis an enzyme known for its enormous applications for industry and diagnostics. Their basic activity is to convert fats into fatty acids and glycerol. These enzymes are water soluble in nature. They also convert polar solvents into more lipolytic substances.
Biochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining.