SIM tube was used as well as the Triple Sugar Iron (TSI), MacConkey agar (MAC) and Citrate Slant. The SIM tube is used to identify hydrogen sulfide production, indole, which is a by-product of tryptophan which is broken down by tryptophanase and motility. The streaking technique used is a half stab. The TSI has an orange color and it used to identify carbohydrate fermentation specifically glucose, lactose, and sucrose fermentation. The TSI is used to observe the slant and butt of the tube as well as to identify if gas was present and if the organism produced hydrogen sulfide.
Abstract This experiment was carried out to determine the species of the unknown organism. Once a choice of the unknown was made a Gram stain was conducted to determine the gram nature and morphology of the organism which was Gram negative bacilli. Based on those results a citrate utilization test was performed resulting in a positive test. Following the flow chart the next test to conduct was a motility test which also had a positive outcome.
Inoculate the test agar medium: There are two types of inoculation that can be done. Phenol Red Broth – Glucose, Lactose, Sucrose The test results are as follows: for glucose, lactose, and Unknown 361 tested A/-, meaning that acid was produced, but no gas and it tested K for sucrose meaning that there was alkaline production. Procedure: 1.
It takes non-acidic potassium iron out of the stomach and replaces it with an acidic hydrogen ion, which makes things acidic. By putting more hydrogen ions into the stomach, the pump makes its contents more acidic. But acid secretion into the stomach stops when a person takes a proton pump inhibitor that stops the proton pump from working. PPIs stop cells in the lining of the stomach producing too much acid. This can help to prevent ulcers from forming or assist in their healing process.
The glucose in your blood comes from carbohydrates in your food. Carbohydrates include sugar and starchy foods like: bread, pasta and rice. Keywords: Carbon dioxide, Glucose, Water, Oxygen and energy.
Bacterial transformation is important because it allows for the cloning and movement of DNA between strains. This transformation usually occurs within plasmids, which are closed circular molecules made up of double stranded DNA. The function of the plasmid is to provide bacteria with genetic advantages such as antibiotic resistance. In this lab, the plasmids provided the ampicillin resistance and the fluorescence. If the bacterial cells are grown in the presence of the antibiotic ampicillin then only the cells that took up the plasmid have the resistance gene.
People on gluten free diet or those with severe allergies should be careful because xanthan gum is produced by bacterial fermentation of a sugar-containing medium which is often a potentially allergenic substance such as soy, corn, wheat or dairy and an allergic response may be triggered in people exceedingly sensitive to the growth medium. Although, the vast majority of industrially manufactured xanthan gum contains far less than 20 ppm gluten, which is the limit for "gluten free" labelling in Canada, United States and Europe. Since information about producing practice is not available, you can check with the manufacturer to find out more info on how it is produced or you
Photosynthesis is, in a nutshell, a chemical process through which plants, some bacteria and algae, produce glucose and oxygen from carbon dioxide and water, using only light as a source of energy. In this experiment, we saw that even underwater plants (Elodea) respire, by exchanging carbon dioxide with the water, in the form of Carbonic Acid. When CO2 is used in photosynthesis, the level of Carbonic Acid drops. Thus, water living organisms have the ability to change the pH of their environment as they add or remove CO2. Bromothymol Blue was added to the test tubes as a neutral pH of 7.0.
Explain the relationship between the ionisation of amino acids and pH |Structural diagram of the neutral structure| |Structural diagram of positively charged structure| |Structural diagram of negatively charged structure| Explain how the form of an amino acid, whether positively charged, negatively charged or neutral, depends of the pH of the solution ? If you increase the pH of a solution of an amino acid by adding hydroxide ions, if this is done then the hydrogen ions will then be removed from the -NH3+group . . To test if it is now a negative ion a process called electrophoresis.although it is colourless its position can be detected using ninhydrin. If the amino acid has dried and then heated gently it would appear as
Catalase activity test establishes whether the bacterium produces the enzyme catalase. The eosin methylene blue test or EMB, inhibits the growth of gram positive bacteria and tests whether or not gram negative bacteria can ferment lactose. Lactose fermentation testing is done to see if the bacterium is capable of fermenting sugar by testing for acid and gas production. These are the possible tests that are needed in order to identify unknown
An intracellular blood buffer like hemoglobin is used because it binds well with hydrogen ions and carbon dioxide. The venous blood, or hemoglobin that isn’t saturated with oxygen, is a better buffer than arterial blood. The phosphate buffer system is important because it regulates the pH in the cytosol. Dibasic phosphate and ammonia are considered renal buffers.
This catalyst is necessary for an aerobic organism to survive in aerobic conditions. After the experiment it was observed that my Gram positive organism did in fact bubble and utilize catalase. The third test conducted was on the MSA agar. This test was conducted for the purpose of selective and differential whether or not my organism can tolerate high salt concentrations. It is based on the mannitol fermentation.
This concept created a guide on naming all Bacteria and Archaea. The biological species concept on the other hand, does not take physical appearance into account on comparing and contrasting organisms but defines a species as part of populations that potentially interbreeds in nature. Students cannot classify microbes within the guidelines of a