Although it was hypothesized that the corn syrup would also contain starch, conducted research helped to clarify why this substance was void of starch. First of all, it was hypothesized that whipping cream would not contain starch, and this was confirmed when drops of an iodine solution was added to the test tube. No evidence of starch was observed, but there was a colour change from a white liquid to a yellow hue that stayed at the bottom of the test tube. However, the whipping cream simply took on the colour of the iodine solution, so there was no change that occurred that could have proved a chemical reaction. The reason why whipping cream does not contain starch is due to the fact that starch is found in plants.
The technique is often used in research to detect specific proteins which have extracted from cells. In this process, a mixture of proteins separated based on two distinguishing properties which are molecular weight and antibody binding specificity. According to the procedure, proteins first separated based on size which have to perform with SDS-PAGE. Next, the proteins from the gel are then transferred to a polymer membrane (PVDF or nitrocellulose) to make them more accessible to the antibodies that specific to the target protein. Cytotoxic assay Cytotoxicity is described as the quality of being toxic to cells.
Inducing Prodigiosin Transposon mutagenesis in Serratia Marcescens Introduction Serratia Marcescens is an opportunistic pathogen, mainly of healthcare facilities but can also be found in many diverse environments. Serratia is a gram negative bacteria which can give it innate resistance to certain antibiotics, especially those that target peptidoglycan cell wall synthesis, due to its outer membrane. In an environment with different microorganisms competing for food Serratia holds a component that gives it another selective advantage. The bacteria contains a red pigment called prodigiosin, that has antibacterial, antifungal, and even antiprotozoal activity. The pigment is produced due to quorum sensing of bacteria, when an appropriate level of N-hex anoyl-L-homoserine lactone (HHL),
6. Drug Resistance Tests These must be executed in focused laboratories to measure the receptiveness to antimalarial compounds of parasites collected from a definite patient. Two main laboratory methods are available: In vitro tests: where the parasites are grown in culture in the presence of increasing concentrations of drugs; the drug concentration that inhibits parasite growth is used as
CH 204 - Introduction to Chemical Practice Experiment 2 - Qualitative Analysis of Cations Petra Hsia Stefi Hsia TA: Joey Gurrentz February 8, 2018 RESULTS & DISCUSSION In Part A of the experiment, the presence of silver was confirmed by the "Unknown 4" substance. It was discovered with two rounds of testing. In the first round, two drops of 6M acetic acid and 4 drops of 1M K2CrO4 was added to the "Step 6" test tube, the solution turned a yellow-orange color. Because there was no formation of yellow precipitate, it was confirmed that lead was not present in the solution. Since lead was not present, the "Part A" test tube, which contained precipitate from the "Unknown 4" substance, was now to be tested for the presence of silver.
A peripheral blood film is a laboratory work-up that involves the cytology of peripheral blood cells smeared on a slide. It focuses on three main blood cells: red blood cells, white blood cells and platelets. A peripheral blood smear is invaluable in the characterization of various clinical diseases. Initiation of a PBF is often a clinical request based on clinical suspicion. It may be initiated based on abnormal findings from an automated count or patients clinical information whose diagnosis maybe supported by a peripheral blood film.
The roots of beet contain an immense quantity of a reddish color called betacyanin that is located inside the central vacuoles, which are surrounded by a vacuolar membrane called the tonoplast (Biology Lab Manual, 2011). The protein structure of beetroot is very important, because once its interrupted it could lose its purpose and would have to experience denaturation. Cell membranes defend and organize cells, they serve as a fence which means that a few particles can disperse across the lipid bilayer but others cannot. The structure of the cell membrane contains
These plates would be those which were treated with RNase, protease, lysozyme and the plate with buffer only. The only plate expected to not have any growth was the plate which was treated with DNase. The DNase would have broken down the double-stranded DNA molecule into its nucleotides and thus, would have been unable to transfer genetic material from the ampicillin resistant strain of E. coli to the ampicillin sensitive strain. Therefore, no new transformants containing ampicillin resistance would have been present and so, the bacteria would have been killed by the ampicillin-containing plate. The experiment conduced at Wits did not correlate with Avery and MacLeod's results entirely.
It favors the skin, since it is part of the normal flora. Its virulence factor comes from multiple things within the cell and these things contribute to the types of infections they cause. Two important virulence factors are a secreted protein called coagulase and clumping factor. Some other virulent factors are the capsule, enterotoxins, exfoliatin, toxic shock syndrome toxin, and alpha toxin. The enterotoxins cause food poisoning in humans.
But what do the antibiotics exactly do? And how they can cure diseases? As a matter of fact, Antibiotics are powerful medicines that can cure bacterial infections if they are used properly. They fight against bacteria by destroying or inhibiting bacteria growth. Soil bacteria and fungi are the natural components of Antibiotics ((n.d.).
This seems to be highly accepted, however it is not indicative of the results that Group 2 obtained. Group 2 used E. coli as the bacteria sample for the UV exposure portion of the experiment. There was no reduction in the bacterial growth on the side where UV light was used as compared to the side that was kept from the UV light. These results, or lack of results, were unexpected and when compared to other groups in lab, appeared to be inaccurate. It appears that Group 2 's UV light did not work.
This includes running the blood the test the antibodies against proteins found on the Lyme disease bacteria. A spinal tap is also performed for patients that experience nervous system problems. This test also looks for antibodies that are against the Lyme disease Bacteria.
No quick endospore stain was performed to validate this assumption since only one assigned organism was endospore forming and unlike Unknown #10, that organism was Gram positive. By Gram staining alone, it was safe to eliminate the three Gram positive bacteria that could have been assigned: S. epidermidis, M. luteus, and B. megaterium. The second step was to streak plate Unknown #10 to observe its macroscopic
Our last petri dish was minus DNA and lysogeny broth, in this petri dish we saw 7 colonies of bacteria and there were a light yellow color. These results mean that we didn’t infect that petri dish with ampicillin from the pipette. Our expectations were both unsupported and confirmed. For example, our minus DNA and lysogeny broth had bacteria growth while our LB/amp/ara plus DNA dish had no growth and did not glow. Some problems that might have occurred in the lab is that we either put too much ampicillin in our LB/amp/ara dish or we could have had all plasma and no bacteria.
The normal flora will compete with the foreign bacteria for nutrients and space and has the ability to push out or starve the invader as said in class. In the article, Skin Microbes Help Clear Infection by Anna Azvolinsky, she talks about an experiment conducted by Stanley Spinola of Indiana University whose goal was to analyze the type of ecology found on the human skin and observe the effects it has regarding the skin 's