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Caaulobacter Crescentus Case Study

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The paper chosen for this assignment, written by A. Persat et.al, was published on 2014 in Nature Communications and is titled ‘The curved shape of Caulobacter crescentus enhances surface colonization in flow’. It essentially ascertains functionality for the prevalent curved shape seen in C. crescentus. The authors discovered that this curved shape offers the bacteria a selective advantage by allowing it to colonise better in environments with water flow. Thus the shape in this case does have a function: to modulate attachment rate of progeny cells and determine the ability to form microcolonies in flow.

In nature, C. crescentus usually adopts a curved shape attributed to the presence of a cytoskeletal protein called crescentin (CreS). But when creS mutants which have a straight shape, were grown in laboratory conditions they did show any visible defect in growth rates. This raised the question: Does the commonly curved shape confer any selective advantage to the bacteria? The answer is apparently yes.

In their natural environment which is freshwater lakes and streams, C. crescentus experience moderate flow. Under these conditions experiments suggest that the curved shape helps the bacteria to attach and grow better on a surface. The pili which is usually used for motility, in C. crescentus seems to have been re-purposed for …show more content…

At high flow rates interestingly the attachment probability of both the WT and the mutant is almost the same. This is because at high flow rates, mutant straight cells bend due to pressure of the flow increasing their likelihood of attachment. Thus this highlights that it is not a difference in attachment strength but rather the shape which is the primary factor for increasing probability of attachment of curved cells. Also given this data, it would have been better if the title specified that the advantages of WT colonization are limited to low or moderate flow

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