Introduction In the past, it has been vital to distinguish the identities of microorganisms in the world. Knowing their identity has aided in diagnosing numerous diseases and has discovered the most beneficial treatment. Lately, it has been vital to differentiate the identities of microorganisms worldwide. Recognizing their identity has aided in diagnosis numerous diseases and has discovered the most beneficial treatment. To study in microbiology, it is crucial for us to use microscope for microorganism observation. The microscope is an optical instrument that uses a lens or a combination of lenses to produce magnified image of small object. A light microscope can magnify images up to 1000x while electron microscopes can even magnify images more than 100,000x (Nester, 2001). As most of the microorganisms are transparent, staining techniques are used to make them visible such as simple stains and differential stains (Edward A. Birge, 1992). …show more content…
At the end of experiment, we learned how to describe microorganisms' morphology. Material …show more content…
There are some kinds of characteristic such as coccus, bacillus, and spiral. The example of coccus could be such as Staphylococcus aureus, streptococcus and bacteria types-Gram stained. We can observe that these bacteria have purple colour because these bacteria having peptidoglycan to their cell wall. In Gram staining, the outer lipid-based membrane of gram-negative bacteria is removed by an alcohol solution. The alcohol also decolorizes the exposed peptidoglycan layer by dissolving away the previously applied crystal violet. A counterstain (safranin or fuchsine)is then added which recolorizes the bacteria red or pink. Then, the example for bacillus could be mixed bacillus-Gram stained. For the spiral can be
Methods Unknown microbial #398 went through several of tests in order to identify its characteristics when isolated from a urine sample of Doris, a 64- year old patient with a kidney infection. To identify unknown #398, must prepare a working and a reserve stock by the inoculation from a broth culture and by quadrant streaking method on a PEM and EMP plates. The following test procedures were incubated at 37°C for 48 hours for observation and identification for unknown #398. The identification of unknown #398 followed test procedures from Brown1.
Identification of bacteria within Unknown Culture #21 In this experiment, an unknown culture of two different types of bacteria was assigned to each person, a number of tests were performed to isolate and identify these bacterial cells. Based on knowledge from the previous experiments completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially will narrow down the types of tests certain bacteria will and will not respond to. In addition to the gram stain, some of the tests that were used include, a catalase test, an Eosin methylene blue (EMB) agar test, a bile esculin test, and a 6.5% sodium chloride (NaCl) test.
Mannitol Salt Agar (MSA) plate, MacConkey agar (MC) plate, Eosin Methylene Blue agar (EMB), and Hektoen Enteric Agar (HEA) (3). The MacConkey agar plate and the Mannitol Salt agar plate are both used in the identification of the unknown. The MC plate is a selective and differential medium. It is considered a selective medium because the bile salts and crystal violet aspect of the medium prevent the growth of gram positive bacteria (3). This medium is differential because of the lactose and neutral red.
These microorganisms are used to teach us how multicellular organisms came to be and how they can survive today. These small, microscopic organisms are so unique that the identification of them is paramount in the advancements of science. Knowing the chemical makeup, the shape, and the biochemical processes is important in identifying these organisms to understand how they survive and where. A number of tests can be ran on an unknown bacteria to determine their ideal
Although microscopic single-celled organisms inhabited earth long before humans evolved from their primate ancestors, they continue to coexist and coevolve with humans today, flourishing as both harmless and deadly companions. Within her literary work Deadly Companions: How Microbes Shaped Our History, microbiologist Dorothy Crawford begins with a dramatic account of severe acute respiratory syndrome (SARS), the first pandemic of the twenty-first century. Crawford travels back in time four billion years ago to the origin of microbes, recounting the evolutionary history of microbes, showing how microbes spread and cause epidemics, and revealing how coevolution yields host resistance. Furthermore, Crawford explores the intertwining history of microbes and humans, with the purpose to reveal the link between the emergence of microbes and the cultural development of man.
Being able to identify unknown microbes from systematic testing is what makes the field of microbiology so important, especially in infectious disease control. Using the testing procedure laid out by the microbiology field we are able to identify unknown bacteria present in our everyday lives, and along the way learn a lot about their characteristics that separate them from other types of bacteria. Being able to do this is vital in order for us to understand why microbes are present in certain places, how they are able to grow and what restricts their growth, that way they can be combatted if necessary. These techniques for determining unknowns are also important for isolating and testing infectious disease microbes in order to prevent spreading. Another important aspect of being able to identify unknown microbes is the
The unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not
Obtain a small sample of the red epidermal cells from the stalk of the rhubarb by carefully peeling away the layer with forceps. Prepare a wet mount slide of the rhubarb tissue in distilled water only. View your slide under low power on your microscope, and then switch to high power. Draw a diagram of the field of view, and label.
INTRODUCTION Infection Prevention and Control (IPC) is one of the most important agents in the prevention of hospital acquired infections or what we termed nosocomial infections. IPC channels every member of the hospital, which includes, healthcare providers (HCP), patients and the hospitals perse. It is important to practice IPC commandment to every hospital as well as community. The Palestinian Ministry of Health (MOH) adopted the national IPC protocol.
The Another medium used was MAC, it is used to isolate and differentiate gram-negative organisms and it is a pink, dusty rose color. Lastly, the Citrate Slant is a green color and it was used as a differential test to examine enzymes. The media were inoculated at 37°C for 48 hours, then it was observed to determine the
The sterile cotton swab was inserted in the S. epidermidis culture and twirled around to obtain a specimen. The entire plate was inoculate with the swab from top to bottom, to achieve a lawn of growth. The dry forceps was used to remove the antibiotic disk into the appropriate spot on the plate. This process was repeated for the all antibiotics with aseptic technique being used. The plate was incubate with lid up on the bookshelf at room temperature for 48 hours.
After experiment on microscope under oil immersion, I learned that my Unknown is gram positive. Under the lens, the bacteria appears in purple color. Its morphology is cocci arranged in cluster. However, during decolorizing process, I put too much alcohol to the crystal violet-iodine complex making the color overly removed. That led to the result of my gram positive has slightly redish
Bacterial growth is the asexual reproduction, or cell division, of a bacterium into two daughter cells, in a process called binary fission. Providing no mutational event occurs the resulting daughter cells are genetically identical to the original cell. Hence, "local doubling" of the bacterial population occurs. Both daughter cells from the division do not necessarily survive. However, if the number surviving exceeds unity on average, the bacterial population undergoes exponential growth.
Fungi, is also an example of microbial life. They are unicellular or multicellular eukaryotes and are made up of a mass of threadlike hyphae forming mycelium. The cell wall are made from chitin. A mushroom is an example of
A microorganism is a small individual life form. The first microorganism was discovered in 1673 by Antonie Van Leeuwenhoek. He observed the first single celled organism with his microscope. Previously, the existence of microscopic single celled organisms were unknown. In 1854, Louis Pasteur discovered microorganisms that change sugar to lactic acid, causing his wine to spoil.