Catalase Essays

  • Catalase Test

    920 Words  | 4 Pages

    Catalase Test, in this test the microbial culture from Nutrient Agar plates were used. This test determines the production of catalase by the microorganisms. Catalase is an enzyme which decomposes hydrogen peroxide to water and oxygen gas thereby, protecting the microorganisms from the lethal effect of hydrogen peroxide which is accumulated as an end product of aerobic carbohydrate metabolism. (Bahrami-Hessari et. al. 2014) The slide containing Micrococcus luteus produced bubbles when 3% hydrogen

  • Catalase Activity

    730 Words  | 3 Pages

    enzyme activity Exploration: Introduction: Catalase is an enzyme normally found in many plant and animal tissues. Its purpose is to destroy toxic substances like hydrogen peroxide which is a byproduct in many cellular reactions. In this lab, we will use a catalase solution from yeast and determine the effect of substrate concentration on the action of this enzyme. The substrate of the enzyme will be different concentrations of hydrogen peroxide (H2O2). Catalase works by the following mechanism : 2 H2O2

  • Catalase Experiment

    1443 Words  | 6 Pages

    The aim of the investigation was to determine the effect of pH on the rate at which catalase decomposes hydrogen peroxide and consequently answer the researchable question “How does pH influence cells and consequently an organism”. The hypothesis, “As the pH deviates from 7 the initial rate of oxygen production will decrease” is supported by the results. The trend displayed in Figure 3 is, as the pH deviates from 7 the initial rate of reaction decreases. Figure 3 shows that the rate of reaction (%O2/s)

  • Catalase Lab

    1410 Words  | 6 Pages

    Catalase Activity on Substrate Based On Gas Pressure Production Rate Name of the Class Author’s Name Date Enzymes are organic compounds which act as catalysts and speed up biological reactions in biological organisms. They are not destroyed or changed during the reaction but rather they are used over and over again to catalyze many more reactions. Their activity may be affected and altered by factors such as temperature, substrate concentration, enzyme concentration and Ph. Example of enzymes

  • Catalase Reaction Paper

    1275 Words  | 6 Pages

    Results Figure 1. Effect of temperature on the reaction rate between catalase and H2O2 Figure 1 shows that the optimum temperature for catalase to catalyze hydrogen peroxide is around room temperature (30℃) as it has a very fast reaction rate (5). The overall trend is that temperatures that differ from 30℃, will decrease the reaction rate. Discussion This experiment supported the hypothesis, since catalase was the most effective with hydrogen peroxide when it was in an environment with a temperature

  • Enzymes In Human Catalase

    762 Words  | 4 Pages

    extensively in organisms that live in the presence of oxygen, catalase prevents the accumulation of and protects cellular organelles and tissues from damage by peroxide, which is continuously produced by numerous metabolic reactions. Its purpose is to break down hydrogen peroxide, which is a waste product in cellular activity and are poisonous to cells. If it were not for Catalase, the hydrogen peroxide will build up in the bodies and kill us. Catalase speeds up the decomposition of hydrogen peroxide into

  • Catalase Enzyme Lab Report

    1303 Words  | 6 Pages

    more substrates turned into products. The ‘Catalase’ enzyme that was used during this experiment was obtained from peroxisome found in celery which are organelles found in bacteria, plant, and animal cells. It is involved in the breaking down of certain substances and the diminish of reactive oxygen species and that includes hydrogen peroxide (H2O2) which can be a byproduct of the metabolism of oxygen. Hydrogen peroxide is toxic to the cell and so the catalase enzyme is utilised to break down H2O2 to

  • The Biological Synthesis Of An Enzyme Catalase

    306 Words  | 2 Pages

    substances to become denatured. When the PH changes, the enzymes stop working, and increasing the temperature will cause a permanent change to the shape of the active site, overall causing the enzyme to no longer have the ability to speed up reactions. Catalase (enzyme) is a common enzyme that is found in all living things. This enzyme catalyzes the decomposition of hydrogen peroxide (substrate) into water and oxygen when it is not denatured. The enzyme substrate complex is when the enzyme and substrate

  • Lab Report On Catalase And Temperature

    899 Words  | 4 Pages

    Catalase and Temperature Introduction Background: Enzymes are catalysts which help reactions inside of organisms such as cells. Many different types of enzymes are used to catalyze different types of reactions. Enzymes are able to catalyze reactions that normally wouldn’t be possible under the specific circumstances in the cell such as the pressure or temperature of the cell. The way an enzyme works is it binds with the active site of a substrate and creates an enzyme substrate complex. The enzyme

  • Enzyme Literature Review

    842 Words  | 4 Pages

    Literature review Research question is how different temperatures affect the catalase enzyme. What is an enzyme? Enzymes are macromolecular biological catalysts. Enzymes speed up chemical reactions. Substrates are molecules that enzymes could act upon and the enzyme converts the substrates into different molecules known as products. Enzyme catalysis is needed in almost all metabolic processes in order to happen in rates/ways that are fast enough to sustain life. (wikipedia. 2018. enzyme. [ONLINE]

  • Enzyme Lab Report Essay

    804 Words  | 4 Pages

    and enzyme catalase and water, the catalase is used to break down the Hydrogen peroxide and the oxygen in the water. You will be able to see as the oxygen produce in the reaction chamber and travel through the hose and up the graduated cylinder. We are going to capture the oxygen gas that being produce in the reaction chamber and see how

  • Enzyme Catalyzed Reaction Lab

    625 Words  | 3 Pages

    rate of reaction Leong, M., Kim, E., Nair, A. Achilly, K., 9/22/2015 Introduction: An enzyme is a protein that acts as a biological catalyst. A catalyst increases the rate of reaction by reducing the activation energy required (Reece 2005). Catalase, an enzyme produced by most living organisms, catalyzes the decomposition of H2O2 in our bodies in order to maintain homeostasis. Enzyme activity involves the binding of an enzyme to a substrate at its active site. Each active site is different and

  • Catalase Experiment

    1574 Words  | 7 Pages

    What is the effect of temperature on oxygen gas production in a Bos taurus liver catalase reaction, with substrate hydrogen peroxide, measured by a gas pressure sensor? Biological catalysts called enzymes are made by living cells and increase biochemical reactions that take place. Enzymes are globular proteins having a multiplex 3-dimensional structure, can increase the rate of chemical reactions without themselves being changed. Enzymes transform substrates into a product. Enzymes have a region

  • Catalase Essay

    724 Words  | 3 Pages

    3.6.4 Assay of Catalase (CAT) Catalase activity was assayed by measuring the inhibition rate of Hydrogen peroxide at 240nm according to the method described by Luck (1974). For this assay, • A 20% homogenate of the leaf extracts of different plants was prepared in phosphate buffer, 0.067 M (pH 7.0). The homogenate was then centrifuged. The supernatant was then used as enzyme extract. • Hydrogen peroxide (H2O2, 2mM) in phosphate buffer (3.0ml) was taken in an experimental cuvette, followed by the

  • The Importance Of Enzymes

    1434 Words  | 6 Pages

    which they are surrounded by is increased. This explains why increasing the temperature of the catalase reaction would increase the rate of reaction. Another requirement for successful collision other than overcoming the activation energy barrier is having the correct geometrical orientation. My prediction can also be supported by the fact that faster movements of hydrogen peroxide molecules and catalase molecules will increase collision frequency, which will ultimately increase the number of successful

  • Enzyme Concentration Lab Report

    1515 Words  | 7 Pages

    The dry yeast is dissolved in water become a mixture and filter paper discs are dip into this catalase solution. Then, the discs would contain catalase and be put into the bottom of the hydrogen peroxide where the chemical reaction would take place. Because of catalase, the reaction would take place quickly and oxygen, one of the products of the reaction, would stay on the surface of the filter paper disc making its density to decrease

  • Potato Enzyme Lab Report

    1182 Words  | 5 Pages

    experiments were performed that pertained to the enzyme known as catalase, which converts hydrogen peroxide into oxygen. Due to peroxide being toxic to the tissues of both plants and animals, both possess the enzyme catalase, which breaks into two non-toxic compounds: water and oxygen gas. Enzymes are proteins that react to certain substrates to create a product, and continue doing so afterwards. Methods and Materials To test reactions between catalase and hydrogen peroxide, groups of three to four people were

  • How Does Temperature Affect The Temperature Of Hydrogen Peroxide

    1776 Words  | 8 Pages

    reaction that occurs by the catalase enzyme within hydrogen peroxide? If the temperature of the hydrogen peroxide is increased to 40° or more the catalase enzymes reaction speed (time it will take for the filter paper to float to the hydrogen peroxides surface in the test tube) will drastically decrease and ultimately the enzyme will become less effective. Independent. The temperature of the hydrogen peroxide. Dependent. Speed ( in seconds) or the reaction rate of the Catalase enzyme that occurs within

  • Tannase Agitation Rate

    2261 Words  | 10 Pages

    Agitation rpm level tannase production Agitation rate was at a range of 50 to 250 rpm level was chosen to determine the optimal rate. It was found that an agitation rate of 100 rpm at pH 6.0 and 37°C maximum yield of tannase production of 3.12 U/ml (Fig.4) it was also noted that an increase in agitation speed above100 rpm resulted in a drastic fall in tannase enzyme production. The agitation speed below 100 rpm level resulted in an inadequate mixing of the broth towards of the broth. Towards the

  • Enzymes: Biological Catalysts

    2446 Words  | 10 Pages

    An enzyme can be regarded as a catalyst for a biochemical reaction, or more simply, a biological catalyst. Its sole purpose is to increase the rate of a reaction, or speed up the reaction via provision of an alternative reactive pathway, which entails a lower activation energy. Enzymes participate in the reaction itself, enabling the occurrence of an alternate pathways of reaction, but they do not attain permanent changes to their structure or nature, and as a result remain unchanged preceding a