Antir Spectroscopy Lab Report
FTIR Spectroscopic Study on Quantitation of Urea in Human BloodSerum
Abstract: The quantitation of urea has been achieved using FTIR spectroscopy. The FTIR spectra of human blood serum samples are recorded in Mid IR region 4000-400 cm-1.The normal blood serum is treated with urea at different concentrations and FTIR spectra are recorded, which confirm the specific peaks related to urea. A plot between concentration of urea and percentage of absorbancehas shown linear relationship. The study being complementary to chemical analysis is very much useful for the estimation of urea in the blood serum of patients suffering from diabetes and renal diseases.
Key Words: FTIR Spectroscopy, Quantitation, Human blood, Serum, Urea.
INTRODUCTION: IR spectroscopy has been used by Biophysicist and Chemist as a powerful tool to characterize
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2. MATERIALS AND METHODS:
The blood samples were collected from healthy donors without adding anticoagulant and serum was separated by removing blood clot. The serum was treated with research grade (SD Fine Chem) glucose at the concentrations of 100,200,300,400 and 500 gm/dL. Infrared spectrum was recorded in FTIR spectrophotometer (ShimadzuFTIR - 8400S) in the range of 4000 cm-1 to 400 cm-1. The resolution was kept at 4 cm-1 and scanning time was fixed at38 Sec. A total number of 10 scans were carried out on each sample.
3. RESULTS ANDDISCUSSION:
Fig.1. shows FTIR spectra of human blood serum treated with Urea of concentrations 100, 200, 300, 400, 500 mg/dl. Spectra show a series of bands, in the range of 4000 cm-1 and 400 cm-1, related to functional groups of proteins, lipids and carbohydrates and other inorganic materials present in the blood serum. Characteristic band for Urea can be seen approximately at 1085 cm-1.
Wave Number (cm-1)
Fig.1.(a). FTIR spectrum of human blood serum treated with Urea of concentration
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