Bioactivity guided fractionation of potent anti-acne plant extract against Propionibacterium acnes 1. Vinod Gauttam Department of Pharmacognosy, ISFcollege of Pharmacy, Moga, Punjab, India * vinodgauttam@gmail.com 2. Nitisha Negi Department of Pharmacognosy, ISFcollege of Pharmacy, Moga, Punjab, India * nitisha.negi@gmail.com 3. Malika Arora Department of Pharmaceutics, ISFcollege of Pharmacy, Moga, Punjab, India * maliksmonu@gmail.com 4. Kavita Munjal Department of Pharmacognosy, ISFcollege of Pharmacy, Moga, Punjab, India * kavitamunjal915@gmail.com ISF College of Pharmacy, Moga, Punjab, India ABSTRACT Background: Propionibacterium acnes (P. acnes) are a commensal anaerobic, Gram positive bacterium that belongs to the normal microflora. P. acnes plays an important role in the pathogenesis of various skin infections and diseases. But the available agents are associated with number of side effects and P. acnes show the genetic base of antibacterial resistance against erythromycin and clindamycin. Therefore, alternative natural approaches for the treatment are needed for alleviation of infection caused by P. acnes. Objective: To find out the most effective anti P. acne extract amongst the selected four plants, commonly used for anti-acne potential and to obtain the characterized fraction of the extract with highest anti P. acne potential. Method: …show more content…
S.K. Srivastava, Scientist D/HOO, Botanical Survey of India (B.S.I), Dehradun (Ref. No. BSI/NRC/TECH (Ident.)/2013-2014/ 1032). The Tephrosia purpurea (leaves) were procured from Tirupati, Andhra Pradesh and authenticated by Dr. K. Madhava Chetty, Associate Professor, Department of Botany, Sri Venkateshwara University, Tirupati, Andhra Pradesh,
After lawn inoculating a Meuller Hinton plate and placing the samples of medication, the plate was then incubated for one week at 37 degrees Celsius. The first medication choice was Trimethoprim, this produced a zone of inhibition of 16mm, therefore being sensitive to the bacteria. Antibiotic number two was nalidixic acid, this too, has a zone of inhibition of 16mm but is considered intermediate. The next antibiotic was erythromycin which produced a zone of inhibition of zero and was therefore resistant. The last antibiotic that was chosen to be used in the experiment was ciprofloxacin.
The summary for the book begins with our main character Zits waking up in a new foster home. His mother died of breast cancer when he was six and his father abandoned him minutes after he was born. Zits doesn’t like his new foster parents and when he attacks his foster mom and runs, he runs into Officer Dave, a police officer in Seattle who has arrested Zits on multiple occasions. Officer Dave sends Zits to juvenile hall. While in the holding cell, he encounters a kid around the same age as him named Justice who he gets along with and befriends him.
Materials and Methods To start with, the unknown bacteria # 710 broth had to be successfully isolated on an EMB and MAC agar plate. Using aseptic technique by sterilizing the wire loop with Bunsen Burner between inoculations and flaming the opening of the test tubes before inserting in the loop with the bacteria. The streaking technique used was to isolate the colonies on the agar plates. In addition, the streak plates had to be incubated in a upturned position for 24 hours in a hot temperature incubator at 37 degree Celsius. Bacteria need a favorable condition to grow in.
Escherichia coli (E. coli) is normally found in living intestine of humans and animals. A bacterium that are harmless and is important to the health of the human’s intestinal tract. The intestinal tract is part of the digestive system; having varies of sizes of intestine that transport the food in order to absorb the nutrients and expel the waste. However, some E. coli are pathogenic, causing illness to the host such as diarrhea or harm the outside of the intestinal tract. The host can be effected by the consumption of contaminated water or food, or having contact with the animals or people.
Name: Rita Thomas Causative Agent: Clostridium perfringens Disease: Gas Gangrene, food poisoning Classification of the causative agent: C. perfringens is a gram-positive, rod shaped, anaerobic, spore-forming, pathogenic bacterium of the genus Clostridium. It is usually present in nature and often can be found in components of decaying vegetation. The spores of the organism persist in soil, different types of sediments, and areas that are subject to human or animal fecal contamination. It frequently occurs in the intestinal tract of humans and other vertebrates, insects, and soil.
Introduction And Background We perpetually worry perpetually concerning skin disorder and it ought to be this fashion too. nobody would ever need spots and pimples on their face, particularly on their face! which region is that space wherever skin disorder appears to hit the foremost. Our face is constantly exposed to everything around whereas most of the body has the shelter of an article of clothing. It meets the wind, the dust, the humidness and conjointly the utmost exposure to daylight that all told cases are that the hardest issue for skin disorder.
Being able to identify unknown microbes from systematic testing is what makes the field of microbiology so important, especially in infectious disease control. Using the testing procedure laid out by the microbiology field we are able to identify unknown bacteria present in our everyday lives, and along the way learn a lot about their characteristics that separate them from other types of bacteria. Being able to do this is vital in order for us to understand why microbes are present in certain places, how they are able to grow and what restricts their growth, that way they can be combatted if necessary. These techniques for determining unknowns are also important for isolating and testing infectious disease microbes in order to prevent spreading. Another important aspect of being able to identify unknown microbes is the
Arwa Al Meslat English 211 September 10, 2014 Ms. Lindsay Gail O`Dunn Vitiligo Have you ever saw someone with white spots?. This may occur because of a skin disease. There is a large number of skin diseases related to the change of the skin color like vitiligo. It is well known that many people do not have enough information about vitiligo, or about its symptoms. In the world population there is 1 of 200 suffers from vitiligo, which means 40 million people, or about 1-2% from the world population*.
Acne Diet: Healthy Eating = Healthy Complexion Science has yet to explain the direct relation of acne and a person’s diet but evidences are starting to pile with regard to the pros and cons of eating particular kinds of food. Based on some clinical researchers, it seems justifiable to consume a low-glycemic diet that is filled with lots of vegetables and fruits. Omega-3 fatty acids are also a must in a person’s diet, whether or not that person has acne or not.
aureus microbe problematic is because it is the leading cause of nosocomial or hospital-acquired infections by gram-positive bacteria and is notoriously resistant to penicillin and many other commonly used antibiotics. It was actually recently reported that a strain of S. aureus is resistant to every known antibiotic in clinical usage. The emergence of antibiotic resistance strains of S. aureus, such as methicillin resistant staph aureus (MRSA), is becoming a greater problem. Since S. aureus has become resistant to most of the beta-lactam antibiotics, vancomycin, a glycopeptide antibiotic, is used to fight MRSA. However, there are now strains of S. aureus that are resistant to vancomycin, such as vancomycin intermediate staph aureus (VISA) and vancomycin resistant staph aureus (VRSA).
Dermatitis is an inflammation of the skin when the skin becomes dry, red, scaly, flaky, itchy, and can be blistered. The skin becomes hard, thickened and cracked, swelled, especially in the eyes, face, or groin areas. Skin can appear darkened or leathery, extreme itching and sun sensitive. The most vulnerable parts of the body are the hands, the forearms and face.
The world is a wondrous place. From Amazonian Brown-Throated Sloth to the Saguaro Cactus of the Sonoran desert, there is an existential natural beauty that poets have aspired to capture in words and artists since the beginning of time tried to render it in paint. When people usually talk about exotic plants, the first dot that connects in the head is that of immensely beautiful rainforests of ours. Though that is justified but what is usually forgotten are the wondrous desert plants. Their beauty is accentuated by their incalculable and varied use in these regions.
Talking about skin aging is a sensitive topic these days as no one would like to actually picture the whole process of their skin losing that youthful appeal after a few years. However, it is essential to know that our skin is like any other part of our body. To be more scientific, skin is the largest organ of the human body. This organ is the first layer which serves as the line of defense and prevents the entry of harmful microbes as well as harmful particles. Apart from this, the body temperature is regulated and the moisture content in the body is also regulated to a certain extent by the skin.
Taxonomic revision of Rochefortia Sw. (Ehretiaceae, Boraginales). Biodiversity Data Journal 4: e7720.
INTRODUCTION: In this experiment I was testing for antimicrobial sensitivity of Staphylococcus epidermidis by using the Kirby-Bauer Diffusion test. The three antibiotics utilized in this lab were: gentamicin, novobiocin, and penicillin. I determined the effectiveness of the antibiotic by observing and measuring the zone of inhibition for each antibiotic.