Fermentation is widely known as a method to preserve food. Many countries using fermentation to preserve fish, Thailand is between the famous one. There are many fermented foods in Thailand, one of them is a fermented mixture of fish and white rice called "som-fak".Som-fak is produced by spontaneous fermentation using white rice and garlic, preserved at room temperature to achieve new texture, flavour, and odour. The methods in this experiment is laboratory-scale experiment to know the process of making som-fak and it’s characterization. pH value is tested for the product before and after fermentation to determine wether the acidic condition arise or not. Descriptive test is used to determine the quality of the final products. The final products …show more content…
Introduction
As we already know, fish is rich with protein and other important nutrition. Unfortunately, fish is categorized as perishable food. Therefore, people try to preserve it by various method. One way of preserving fish is fermentation. Preserving fish by fermentation is a traditional preservation methods which widely used in Indonesia and Southeast Asian countries, because it is relatively cheap and easy. Fish and seafood are mixed with salt, rice and spices is a traditional fermentation is done in many countries of Southeast Asia. (Paludan-Muller et al., 2002).
One of the famous fermentation products from Thailand is Som-fak. Som-fak is a low salt fermented fish products which fermented for 2-4 days at room temperature and the have dominant microflora namely lactic acid bacteriaor (LAB). Som-fak made from freshwater fish meat mixed with rice, garlic and salt and then wrapped in banana leaves. It is fermented at room temperature to form acidic flavor (Rahayu et al.1992).
2. Material and Methods
The method used is a laboratory-scale experimental methods. Implemented in Processing Laboratory of the Faculty of Fisheries and Marine Sciences, Diponegoro University, in May 2014. Tools and materials used are : Process of Making
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Spontaneous fermentation is a fermentation which the type and number of microbes in the fermentation process are unknown and uncontrolled. Spontaneous fermentation process depends on the type of raw materials and environmental conditions during fermentation. Events that occur during som-fak fermentation are changes in the composition of glucose and fish protein. Glucose converted into lactic acid by microbes. This change is caused by anaerobic conditions applied to the product. Degradation of glucose to lactic acid fermentation is a major process in som-fak where it affects the durable power and quality of the final product. According to Muchtadi and Sugiyono (2013) result of fermentation obtained from metabolism of microbes in product under anaerobic state. Microbial fermentation requires energy which is generally obtained from
A milk-based, litmus broth tube is incubated and observed after 48 hours. Observations include lactose fermentation without gas as well as with gas, the reduction of litmus, casein protein coagulation and casein and protein hydrolysis. These characteristics were all determined based on the color of the solution and the production of a curd, the curds density and the production of a gas. To determine the density of the curd, the tube was slightly turned to see rather or not it was mobile or concentrated towards the bottom. 2.3 Carbohydrate Fermentation of Lactose, Sucrose and
Therefore, acid fat, lactose fermentation, mannitol fermentation were not needed to be performed, because they are selective to a specific thing. As a result, Unknown bacteria “W” was concluded to be gram stain positive, endospore positive, bacilli shape (rod shape), and arranged in chains (strepto-). Test Purpose Reagents Observation Results Gram Stain To determine gram reaction of bacteria.
There are several reactions occur when there is plenty of oxygen present. Then the energy released is used by the yeast for growth and activity. However, when the oxygen supply is limited, the yeast can only partially breakdown the sugar. Alcohol and carbon dioxide are produced in this process known as alcoholic fermentation. The fermentation occur when the carbon dioxide produced in these reactions.
Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable pH 3. Controlled Variables temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time Effect of Temperature on Enzyme Activity 1.
LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.22.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3. Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2.
Title: How Ph Levels Affected the Fermentation of Beer Hypothesis: The beer will be left with more sugar deposit as the Ph levels increase because alpha/beta -amylase will no longer function. Predictions: Alcohol Percentage Analysis for the Control and the Experimental During this experiment, the pH level was increased, therefore Alpha-Amylase was favored. Due to the nature of Alpha-Amylase cutting randomly through a large carbohydrate molecule, it leaves bigger sugars in the flask, which cannot be digested by yeast. Due to this, less reactions should occur in the experimental, therefore leading to a lower percentage of alcohol production, compared to the control.
For example, fermentation occurs in yeast in order to gain energy by transforming sugar into alcohol. Fermentation is also used by bacteria, they convert carbohydrates into lactic acid. Ethanol fermentation is done by yeast and certain bacteria, when pyruvate is separated into ethanol and carbon dioxide. Ethanol fermentation has a net chemical equation: C6H12O6 (glucose) > 2C2H5OH (ethanol) + 2CO2 (carbon dioxide). This process of ethanol fermentation is used in the making of wine, bread, and beer.
What is the effect of temperatures 10°C , 20°C, 40°C, 60°C and 70°C ± 1/°C on yeast fermentation when baking bread? ii. Aim: The focal aim of this experiment is to investigate the effect that temperature has on the growth and respiration of yeast (Saccharomyces cerevisiae) fermentation. iii.
Yeast is alive because it can to metabolize and respond to environmental changes. The purpose of the first experiment was to determine whether yeast can metabolize. The bromothymol blue solution with yeast changed from blue to yellow. Bromothymol blue is an acid-base indicator that turns yellow in the presence of acid. The color change indicates that carbonic acid was formed from the reaction of water and carbon dioxide, a byproduct of metabolization.
A 50 mL buret was obtained and was washed with NaOH solution. After filling the buret with NaOH (titrant) and preparing the KHP (analyte) in the Erlenmeyer flask, the solutions were titrated. The volume used from the NaOH solution was recorded. C. Determination of the Acidity of Soft Drinks First, the soft drinks were heated.
This experiment has to be carried out carefully
Joshua Miller 12/18/17 Fermentation Lab report Introduction The term fermentation refers to the chemical breakdown of a substance by bacteria, yeasts, or other microorganisms, typically involving effervescence and the giving off of heat (wikipedia). Sugars are converted to ethyl alcohol when fermentation happens. In this experiment we determined if yeast cells undergo fermentation when placed in a closed flask with no oxygen. Glucose and yeast are mixed together in a closed flask and allowed to incubate for about one hour.
Biochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining.
According to a lot of Malaysians, there is nothing that symbolises Malaysia better than “mamak” stalls. These stalls serve a variety of mouth-watering food such as the iconic “roti canai” and the infamous “teh tarik”. People of
Abstract — This experiment was conducted to familiarize the students with the procedures regarding distillation—to be more precise, the separation of ethanol from an alcoholic beverage—using a distillation set-up consisting of boiling chips, a Bunsen burner, a condenser, a thermometer and several other materials. In the end, it was discovered that one may actually separate a homogeneous mixture, given that the components of said mixture differ in volatility and that they utilize a complete distillation set-up and follow laboratory safety rules and regulations. Keywords — Matter, homogeneous and hetereogeneous mixtures, distillation, volatility, boiling point I. INTRODUCTION There are typically two categories of matter, these are pure substances