Ensured the complete dissolution of Agarose by checking against the light. 3. Cooled the solution to 50°C and added 4.5 l of ethidium bromide dye to a final concentration of 0.5g/ml and mixed thoroughly. 4. Slowly poured the warm Agarose solution into the casting tray and inserted the required combs.
The resolution of the DNA changes with the percentage concentration of the gel. Increasing the agarose concentration of a gel reduces the migration speed and improves separation of smaller DNA molecules, while lowering gel concentration permits large DNA molecules to be separated. For a standard agarose gel electrophoresis, a 0.7% gives good separation or resolution of large 5–10kb DNA fragments, while 2% gel gives good resolution for small 0.2–1k fragments • They are the most popular medium for the separation of moderate and large-sized nucleic acids and have a wide range of separation but a relatively low resolving power, since the bands formed in the gels tend to be fuzzy and spread apart. • This is a result of pore size and cannot be largely controlled. These and other advantages and disadvantages of using agarose gels for DNA electrophoresis are summarized in Table below : Advantages Disadvantages • Gels are quick and easy to cast • Nontoxic gel medium • Good for separating large DNA molecules • Can recover samples by melting the gel, digesting with enzyme agarose • Poor separation of low molecular weight samples • High cost of agarose Fuzzy
Bromophenol blue dye- Low molecular weight dye for tracking the progress of electrophoresis. 5. DNA sample. Procedure 2 g agarose was weighed in a conical flask, mixed with TBE buffer (100 ml ) and boiled in a microwave oven to dissolve the agarose and obtain a clear solution. The solution was cooled to a lower temperature and mixed with 2µl of EtBr.
A measured amount of gel (0.5 g) was placed on fixed glass slide with a circle of 1cm diameter; the movable pan with a glass slide attached to it and was placed over the fixed glass slide, such that the gel was sandwiched between the two glass slides for 5min. The increase in the diameter due to spreading of the gel was noted and spreadability was determined using the
Estimation of GeoSpatial Parameter (Lugeon values) using Variogram technique. Abstract: This article gives brief overview on developing Geo-Spatial variational map of parcel of land where few tests has already been carried out. The calculation procedure is demonstrated with dummy Lugeon test values. Such maps may be helpful for practicing engineers who are working in groundwater movement to visualize and estimate the ground water movement. Introduction In Geo-statistics we encounter the situation when there is need to auto-correlate of one or more variables in the space or sometimes in space-time for following purposes: to make estimate at unobserved locations to give information about the accuracy of prediction to reproduce spatial
Using paper towels and 2% solution of gluteraldehyde, wipe down walls of BSC, work surfaces and equipment. 12. With 2% solution of gluteraldehyde, flood work surface and drain pan and let stand for minimum of 20 minutes. 13. Pick up the absorbent material or paper towels.
2:- Front View Fig. 3:- Top view Then by referring the above measurements of the sight, it’s clear from which position the component is picking up and to which position the component is place. Also by using top view measurements calculate the distance required to travel by the arm to place the component. It’s difficult to travel 1000mm distance by arm with the component, so there is requirement to minimize the distance and the optimized position to where the manipulator is placed is in figure no. 4.
After changing the distance of alsthesiometer on 7cm, 0.5cm, 1cm and 1.5cm new trials were carried out. And readings were carried out. In next step the percentages of 1 and 2 point threshold were calculated on longitudinal position In the last step the percentages of 1 and 2 point threshold were calculated on lateral positions. To determine whether the hypothesis has been
MANUFACTURING PROCEDURE (General) Step: 1 – All ingredients were weighed in specified quantity as given in the formula. Step: 2 –Metformin Hydrochloride and Polymer (DCP, HPMC K100M, HPMC K4M, HPMC K15M,) PVP-K30, MCC (Avicel ph-102), Gelatin was sifted through 40# sieve. Step: 4 - The step 2 ingredients(except gelatin) were loaded into planetary mixer and mixed for 30 minutes. Step: 5 – Talc, and Magnesium Stearate was sifted through 40# sieve. Step: 6 –Then a paste was prepared by using 100 ml of DM hot waterand gelatin +(a solution of starch in water previously prepared but in case of IPA granulation where starch and gelatin are not used IPA was added to the step 4 mixture.).