ANALYSIS
REDUCING SUGARS
From the observations obtained within the experiment, it can be concluded that only whipping cream and corn syrup tested positive for reducing sugars. However, it was hypothesized that in addition to whipping cream and corn syrup, the flour solution would also contain reducing sugars. This did not prove to be accurate as when it was tested with the Benedict 's solution, no evident change was recorded. Further research was conducted in order to determine why certain substances tested positive and the reason why others did not undergo any changes. To begin, during the Benedict 's and heat test, the test tube containing whipping cream underwent a colour change from a white, low viscous liquid to a bright orange hue
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Although it was hypothesized that the corn syrup would also contain starch, conducted research helped to clarify why this substance was void of starch. First of all, it was hypothesized that whipping cream would not contain starch, and this was confirmed when drops of an iodine solution was added to the test tube. No evidence of starch was observed, but there was a colour change from a white liquid to a yellow hue that stayed at the bottom of the test tube. However, the whipping cream simply took on the colour of the iodine solution, so there was no change that occurred that could have proved a chemical reaction. The reason why whipping cream does not contain starch is due to the fact that starch is found in plants. Since, cream is not associated with any plants, there is no possibility for starch to exist. Additionally, the nutritional label confirms that there are no sugars present in the whipping cream. Starch is a polysaccharide which is a type of sugar. Therefore, if no sugars are present in the whipping cream, there cannot be any starch as well. Moreover, it was confirmed that soy milk does indeed contain starch. This was also hypothesized as it was assumed that since soybeans are a legume, they would contain starch. The experiment confirmed this result as when the iodine solution was added, the milk turned a dark grey colour at the top of the test tube and when it was mixed, the entire solution became a light grey colour. A blue or black tinted colour is an indicator of starch in a product, so the grey hue that the soybean milk possessed confirmed the hypothesis. Research helped to determine that the type of starch found in soybeans is known as beta-amylase (Stevenson et al., 2006). It is a starch, specified by specific glycosidic linkages, that is commonly found in the stems of plants which would lead to the conclusion that it
Last test, inoculation of phenylalanine agar is used to determine if phenylalanine deaminase oxidizes phenylalanine into phenylpyruvic acid and ammonia. Sixth test, is a Multiple Test Media used to determine the physiological characteristics of unknown #398. First test, Inoculation of Kligler 's Iron agar was used to determine the production of hydrogen sulfide from cysteine and fermentation of glucose and lactose. Last test, inoculation of litmus milk is used to determine the fermentation of lactose, casein, lactalbumin, and
When it comes to the topic of sugar most of us would agree that it impacted the world. Where this agreement ends ,however, is on the question of whether good or bad. Whereas some are convinced that it was a negative change, others maintain that it was a positive change. However sugar affected the world in a negative way by causing slavery, poor work condition, inequality, and low wages.
Starch solution is then placed into the test tube at a quantity of 5 mL. 5 drops of Lugol’s Iodine solution is added to the test tube. If the color changes, then it is known that starches are present in the solution. Proteins are next tested. In order to do this, 5 mL of gelatin solution is added to the test tube. 10 drops of Biuret’s reagent are added to test for protein.
These color changes indicate a chemical change, which show that a reaction had occurred. In the first step when o-vanillin and p-toludine, imine was formed. The color change from green to orange suggests that imine appears as orange colored. In the second step, the addition of sodium borohydride reduced the imine into another derivative, which was yellowish lime color. The solution turned clear when acids and anhydrides was added, which indicated the precipitate were dissolved.
The passing of the Sugar Act further intensified the growing resentment between the colonies and England. George Grenville, the Prime Minister of England, passed the Sugar Act in 1764.This act taxed all of America’s imports. He also more strictly enforced the trade laws. The Americans deeply resented the taxation that they felt was unjust. James Otis put the general mood of the colonists into words when he said each colonist had the right to be “free from all taxes but what he consents to in person, or by his
Macromolecule test 1 differs from the second chart by testing non-reducing sugars in the first test and proteins in the second. In depth the lab required to heat the sample at times, mix them, and add them to a warm water bath of 100 Celsius. The following graphs were obtained by following the guidelines within the
The second question asked was, “Describe how the new mixture is different from the original substances?” The new mixture that was created is different from the original substances because when you would touch the mixture is was a chalky feeling liquid and when you wouldn’t touch it at all it was a solid. From the original substances to the new mixture the cornstarch was very soft and felt like powder sugar and the water was so clear that you could see through it. The third question that was asked was, “Was the new mixture made from a physical change or chemical change? How do you know?”
LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.26.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3.
1% glucose, 1% maltose and 1% lactose all progressively get positive results by changing colours to reddish brown at the end of this experiment. In this case the aldehyde functional group that is present in the products (monosaccharides and some disaccharides) in this reaction is able to reduce copper in the presence of alkali and this produces colour changes while converting to an aldose sugar. Honey is made of fructose and glucose which instantly turned brown after the test-tube was placed in the boiling water because of its active aldehyde and carbonyl group. The copper (II) sulphate present in the Benedict’s solution reacts with electrons from the aldehyde group which results in a redox reaction to from cuprous oxide, a red brown precipitate that seen in all of the above mentioned solutions (Hill, 1982). Beer also gave positive results because it contains aldehydes and ketones (i.e. acetone, trans-2-butenal, furfual) during its beer production process where the sugars are converted through fermentation (Hill, 1982).
Sugar in the Blood (by Andrea Stuart) First Name and Last Name Class Date “Sugar in the Blood” is a book written by Andrea Stuart, female from diverse racial setting. She was born and raised in the Caribbean Island, in particular, the Barbados.
In test tube E, a colourless colour formed. It is because redox reaction occurred during the test. Idoine reduced into idoine ion , which changre from brown to colourless. In test tube F, the iodine solution change from brown to purple . It is because the salt has a function of cofactor which will shorten the time for amylase to take to break down the
Uncontrolled Environmental conditions Atmospheric conditions The controlled variable Concentration of amylase was kept under control by measuring the amount of amylase used and also it was made sure the percentage of amylase used was 1%. The Amount of amylase/starch used were kept to 5cm3 at all times. Materials needed Beakers Bunsen burner Test tube Thermometer Stopwatch Test plate Glass rod Starch Amylase solution Water bath Iodine solution. Test tube holder Labels Marker Procedure First 5 test tubes were taken and labeled with numbers from 1 to
The overall project goals and central questions that has to do with the project is mostly trying to determine the isotonic concentration of the salt in potato roots and the use of the ideal soil salt conditions for the potato plant growth. In part 2, we had to test the enzyme activity that is in the was involved in the potato, so we can also determine the ideal soil pH conditions for the potato plant growth. In part 3, we were able to test absorb the leaf pigment at various wavelengths that determine the optimum light absorption conditions and was able to make recommendations for the light conditions that would be used un greenhouses. The goal of the first project is to determine the ideal soil salt for potato roots and we can relate this to the project is to find out if Solution A or Solution B has more solute in it. The goal of this project is to determine the meaning of Osmosis.
The experiment shall use several concentrations of sucrose solution and a substance known as Methylene blue. A piece of potato/ carrot shall be placed in a boiling tube and the solution shall be poured into it. This tube shall have Methylene blue added into it. After incubation some of this solution shall be taken out with a pipette and inserted into a separate boiling tube containing the same sucrose solution however this solution shall be known as the pre-incubated solution. The drop shall be watched so as to see if the density of the water and concentration of sucrose has increased or not, displaying the water
1.0 Introduction The name of the business that we investigate is Sugarbun. This business provides the consumer the fast food and delivery services. The food provided are vary from sandwiches to broasted chicken to Eastern Cuisine such as nasi lemak classic, sambal eco fish, chicken curry and else and also varieties of beverages. SugarBun is the first Malaysian homegrown fast food restaurant to internationally.